Protective Effects of Platelet-rich plasma for in vitro Fertilization of Rats with Ovarian Failure Induced by Cyclophosphamide

• Published in: Revista Brasileira de ginecologia e obstetrícia 2022-02, Vol.44 (2), p.161-168
• Main Authors: Budak, Özcan, Bostancı, Mehmet Sühha, Toprak, Veysel, Doğanay, Songül, Köse, Osman
• Format: Article
• Language: English
• Subjects: Animals; Cyclophosphamide - toxicity; Female; Fertilization in Vitro; OBSTETRICS & GYNECOLOGY; Original; Ovarian Diseases - chemically induced; Ovarian Diseases - prevention & control; Platelet-Rich Plasma - physiology; Rats; Rats, Sprague-Dawley
• ISSN: 0100-7203; 1806-9339; 1806-9339
• DOI: 10.1055/s-0041-1741451

Premature ovarian insufficiency (POI) contributes significantly to female infertility. Cyclophosphamide (CYC has adverse effects on folliculogenesis. Platelet-rich plasma (PRP) is an autologous product rich in many growth factors. We evaluated the protective effect of PRP on in vitro fertilization in female rats with CYC-induced ovarian damage.  Twenty-eight adult female Sprague-Dawley rats were randomly divided into four groups. Group 1 (control-sodium chloride 0.9%; 1 mL/kg, single-dose intraperitoneal [IP] injection); group 2 (CYC), 75 mg/kg, single-dose IP injection and sodium chloride 0.9% (1 mL/kg, single-dose IP injection); group 3 CYC plus PRP, CYC (75 mg/kg, single-dose and PRP (200 μl, single-dose) IP injection); and group 4 (PRP, 200 μl, single-dose IP injection).  In the comparisons in terms of M1 and M2 oocytes, it was observed that the CYC group presented a significantly lower amount than the control, CYC/PRP, and PRP groups. (for M1,  = 0.000,  = 0.029,  = 0.025; for M2,  = 0.009,  = 0.004,  = 0.000, respectively). The number of fertilized oocytes and two-celled good quality embryos was found to be statistically significant between the CYC and control groups, CYC + PRP and PRP groups (  = 0.009,  = 0.001,  = 0.000 for oocytes, respectively. For embryos;  = 0.016,  = 0.002,  = 0.000).  Platelet-rich plasma can protect the ovarian function against damage caused by CYC, and, in addition, it improves oocyte count and the development of embryos as a result of oocyte stimulation during the IVF procedure.