Pharmacological immunomodulation enhances peripheral nerve regeneration

• Published in: Pesquisa Veterinária Brasileira 2007-09, Vol.27 (9), p.363-369
• Main Authors: Inoe, Ana Paula(Universidade Paranaense), Pereira, Francisco Carlos(Universidade de São Paulo Instituto de Ciências Biomédicas Depto Anatomia), Stopiglia, Angelo João(USP Faculdade de Medicina Verterinária e Zootecnia Depto Cirurgia e Anestesiologia Veterinária), Da-Silva, Ciro Ferreira(USP Instituto de Ciências Biomédicas Depto Biologia Celular e do Desenvolvimento)
• Format: Article
• Language: eng ; por
• Subjects: cirurgia; drug effect; efeitos de drogas; N-acetilmuramil-L-alanil-D-isoglutamina MDP; N-Acetylmuramyl-L-Alanyl-D-Isoglutamine MDP; nerve regeneration; nervo ciático; regeneração nervosa; sciatic nerve; surgery; VETERINARY SCIENCES
• ISSN: 0100-736X; 1678-5150; 0100-736X
• DOI: 10.1590/s0100-736x2007000900002

To assess the effect of N-Acetylmuramyl-L-Alanyl-D-Isoglutamine MDP topically administrated on the regenerating peripheral neurons, twelve male C57BL/6J adult mice were equally distributed into three groups. Four mice underwent unilateral sciatic nerve transection and polyethylene tubulization, with a 4mm gap between the proximal and distal nerve stumps and were implanted with collagen + PBS (COL). Other four animals underwent the same surgical procedure but received collagen + MDP (COL/MDP) inside the prosthesis. Four animals were not operated and served as control group (NOR). After 4 weeks, the regenerated nerve cables were processed for total myelinated axon counting and myelinated fiber diameter measurement. The L5 dorsal root ganglion (DRG) was also removed and sectioned for sensory neurons counting and measurement. The results revealed significant difference (p