Morphometric analysis of the thymus of puppies infected with the Snyder Hill Strain of canine distemper virus

The thymic morphometry analysis was used for determining apoptosis and atrophy of the thymus of eight puppies inoculated with canine distemper virus (CDV). Three healthy dogs were used as negative controls. Sections, 5µm thick, were stained by HE and Shorr, and the latter were evaluated by morphomet...

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Published in:Arquivo brasileiro de medicina veterinária e zootecnia 2006-08, Vol.58 (4), p.472-479
Main Authors: Alves, C.M.(UFMG ICB Departamento de Patologia Geral), Vasconcelos, A.C.(UFMG ICB Departamento de Patologia Geral), Martins, A.S.(UFMG ICB Departamento de Fisiologia e Biofísica), del Puerto, H.L.(UFMG ICB Departamento de Fisiologia e Biofísica), Santos, F.G.A.(UFMG ICB Departamento de Patologia Geral), Nunes, J.E.S.(UFMG ICB Departamento de Patologia Geral), Campos, P.P.(UFMG ICB Departamento de Patologia Geral), Moro, L.(UFMG ICB Departamento de Patologia Geral)
Format: Article
Language:eng ; por
Subjects:
apoptosis
canine distemper
cinomose
cão apoptose
immunology
immunosuppression
imunologia
imunossupressão
puppy
thymus
timo
VETERINARY SCIENCES
virus
vírus
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Summary:The thymic morphometry analysis was used for determining apoptosis and atrophy of the thymus of eight puppies inoculated with canine distemper virus (CDV). Three healthy dogs were used as negative controls. Sections, 5µm thick, were stained by HE and Shorr, and the latter were evaluated by morphometry. CDV nucleoprotein was detected by immunohistochemistry. Morphometric results confirmed lymphoid hypotrophy in CDV inoculated dog thymuses, more stroma, less parenchyma and higher apoptotic index/field than negative control (not inoculated) puppies. Apoptosis plays a role in the mechanism of thymus atrophy that develops in canine distemper. Determinaram-se a apoptose e a atrofia no timo de oito cães novos, inoculados experimentalmente com o vírus de cinomose. Três cães saudáveis foram usados como controle negativo. Secções coradas pelo Shorr foram avaliadas por morfometria. A nucleoproteína viral foi detectada por imunoistoquímica. Os resultados morfométricos confirmaram a hipotrofia e mostraram que o timo dos cães inoculados tinha mais estroma, menos parênquima e maior índice apoptótico/campo que o dos animais-controle. Pode-se concluir que a apoptose desempenha importante papel no mecanismo de hipotrofia tímica que se desenvolve na cinomose.
ISSN:0102-0935
1678-4162
1678-4162
0102-0935
DOI:10.1590/S0102-09352006000400004