Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured

The effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Mediu...

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Published in:Arquivo brasileiro de medicina veterinária e zootecnia 2009-02, Vol.61 (1), p.57-65
Main Authors: Lima-Verde, I.B., Matos, M.H.T., Bruno, J.B., Martins, F.S., Santos, R.R., Báo, S.N., Luque, M.C.A., Vieira, G.A.B., Silveira, E.R., Rodrigues, A.P.R., Figueiredo, J.R., Oliveira, M.A.L., Lima, P.F.
Format: Article
Language:English
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VETERINARY SCIENCES
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Summary:The effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15µM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P
ISSN:0102-0935
1678-4162
0102-0935
DOI:10.1590/S0102-09352009000100009