Histomorphometry of Bone Microarchitecture in Rats Treated with Vitamin D and Bisphosphonate in the Management of Osteoporosis

• Published in: Revista brasileira de ortopedia 2022-04, Vol.57 (2), p.267-272
• Main Authors: Queiroz Júnior, José Reginaldo Alves de, Cartaxo, Marina Falcão de Souza, Paz, Silvania Tavares, Tenório, Fernanda das Chagas Ângelo Mendes, Lemos, Ana Janaína Jeanine Martins de, Maia, Carina Scanoni
• Format: Article
• Language: English
• Subjects: Artigo Original; ORTHOPEDICS
• ISSN: 0102-3616; 1982-4378; 1982-4378
• DOI: 10.1055/s-0041-1741023

To verify how the combined administration of alendronate (ALN) and vitamin D3 (VD) acts on the bone microarchitecture in rats with glucocorticoid-induced osteoporosis.  The experiment used 32 90-day-old female Wistar rats weighing between 300 and 400g. The induction of osteoporosis consisted of intramuscular administration of dexamethasone at a dose of 7.5 mg/kg of body weight once a week for 5 weeks, except for the animals in the control group. The animals were separated into the following groups: G1 (control group without osteoporosis), G2 (control group with osteoporosis without treatment), G3 (group with osteoporosis treated with ALN 0.2 mg/kg), G4 (group with osteoporosis treated with VD 10,000UI/500μL), and G5 (group with osteoporosis treated with ALN + VD). The right femurs of the rats were fixed in 10% buffered formaldehyde, decalcified, and processed for inclusion in paraffin. Histological sections were stained with hematoxylin-eosin for histomorphometric analysis. Cortical thickness and medullary cavity were measured in cross-sections.  There was a statistical difference (