Gastroprotective effect of hydroalcoholic extract from barks of Persea major Kopp (Lauraceae) in rats

• Published in: Revista brasileira de farmacognosia 2007-12, Vol.17 (4), p.533-537
• Main Authors: Cosmo, Simone A., Mayer, Bárbara, Freitas, Cristina S., Baggio, Cristiane H., Marques, Maria Consuelo A.
• Format: Article
• Language: English
• Subjects: PHARMACOLOGY & PHARMACY
• ISSN: 0102-695X; 1981-528X
• DOI: 10.1590/S0102-695X2007000400010

Persea major Kopp (Lauraceae) is used in folk medicine to treat skin wounds and gastric disorders. This study evaluates the potential of crude hydroalcoholic extract (EHA) as gastroprotective and its acute toxicity. Swiss mice were treated with EHA by oral (p.o.) and intraperitoneal (i.p.) routes with doses of 0.125 to 10 g/kg and were observed until 14 days after the administration of the extract. The calculated LD50 of EHA after i.p. administration was 480 mg/kg in mice and the LD50 values of EHA by the oral route were calculated to be up to 10 g/kg in mice. Wistar rats were orally pretreated with EHA (30, 100, 300 and 1000 mg/kg) before induction of gastric lesions by 70% ethanol (0.5 mL/animal, p.o.), indomethacin (20 mg/kg, s.c.) and hypothermic restraint stress (during 3 h at 4 ºC). The EHA protected the gastric mucosa against lesions induced by ethanol, but did not reduce the stress- and indomethacin-induced gastric lesions. When the EHA was injected into the duodenal lumen (i.d.), the volume, pH and total acidity of the gastric secretion of rats with pylorus ligature was not altered with different doses of the extract. Results therefore suggest that the cytoprotective effect of this extract against the direct necrosing action of ethanol and its effect were not related with reduction of gastric acid secretion.