Evaluation of wound healing activity of Ammannia baccifera and Blepharis maderaspatensis leaf extracts on rats

• Published in: Revista brasileira de farmacognosia 2012-04, Vol.22 (2), p.418-427
• Main Authors: Rajasekaran, Aiyalu, Sivakumar, Vellaichamy, Darlinquine, Sabarimuthu
• Format: Article
• Language: English
• Subjects: PHARMACOLOGY & PHARMACY
• ISSN: 0102-695X; 1981-528X
• DOI: 10.1590/S0102-695X2011005000207

Wound healing activity of the leaf extracts of Ammannia baccifera L., Lythraceae, and Blepharis maderaspatensis (L.) B.Heyne ex Roth., Acanthaceae, was investigated by excision and incision wound healing models in rats. A phytochemical screening was done to determine the major constituents of the chloroform, ethyl acetate and ethanolic fractions of ethanolic leaf extracts. The excision and incision models were used to assess the effect of the plant extracts on wound healing in rats. Phytochemical screening reveals the presence of tannins, saponins, steroids, terpenoids, and flavonoids in the extract. The wound healing effect was comparatively evaluated with a standard drug Framycetin cream. Significant wound healing activity was observed for the creams prepared with 5% ethanol fraction of B. maderaspatensis and 5% chloroform fraction of A. baccifera ethanolic leaf extracts. The results of histopathological evaluation supported the outcome of both incision and excision wound models. Ethanolic fraction of B. maderaspatensis and chloroform fraction of A. baccifera exhibited marked wound healing activity. B. maderaspatensis extract displayed a remarkable wound healing activity compared to A. baccifera.