Phenolic-antioxidant capacity of mango seed kernels: therapeutic effect against viper venoms

• Published in: Revista brasileira de farmacognosia 2018-09, Vol.28 (5), p.594-601
• Main Authors: Abdel-Aty, Azza M., Salama, Walaa H., Hamed, Mohamed Belal, Fahmy, Afaf S., Mohamed, Saleh A.
• Format: Article
• Language: English
• Subjects: Anti-venom; Antioxidant; Egyptian mango; Original Article; Oxidative stress; PHARMACOLOGY & PHARMACY; Pharmacy; Phenolic compounds
• ISSN: 0102-695X; 1981-528X; 1981-528X
• DOI: 10.1016/j.bjp.2018.06.008

[Display omitted] In this study, mango seed kernels extract contained a considerable amount of phenolics and flavonoids (17,400 and 3325mg/100g seed, respectively). The HPLC profiling revealed that hesperidin was the major phenolic compound of the mango seed kernels extract. This is the first report find hesperidin in mango extracts. The phenolic compounds of mango seed kernels extract were effective in scavenging free radicals of DPPH and ABTS with IC50 values of 47.3 and 7.9μg/ml, respectively. The total antioxidant activity of mango seed kernels extract based on the reduction of molybdenum was also measured. The phenolic compounds of mango seed kernels extract potentially inhibited the protease, fibrinogenase, phospholipase A2, l-amino acid oxidase, hyaluronidase, and hemolytic activities of the most dangerous Cerastes cerastes and Echis coloratus viper venoms. The phenolic compounds of mango seed kernels extract could completely neutralize the hemorrhage and lethality of both venoms in experimental animals. It could be concluded that the mango seed kernels extract phenolic compounds with potential antioxidant activity are considered as a new avenue in the viper bite treatment.