Pharmacokinetic disposition of erythraline in rats after intravenous administration

• Published in: Revista brasileira de farmacognosia 2019-11, Vol.29 (6), p.773-777
• Main Authors: Demarque, Daniel P., Callejon, Daniel R., Pinto, Larissa G., Gouvea, Dayana R., de Moraes, Natália V., Lopes, João L.C., Lopes, José N.C., Lopes, Norberto P., Guaratini, Thais
• Format: Article
• Language: English
• Subjects: Herbal medicine; Natural products; Original Article; Pharmacokinetics; PHARMACOLOGY & PHARMACY; Pharmacy
• ISSN: 0102-695X; 1981-528X; 1981-528X
• DOI: 10.1016/j.bjp.2019.07.002

[Display omitted] Erythraline is the major alkaloid produced by Erythrina verna Vell., Fabaceae, a plant used in folk medicine and phytotherapeutic products to treat anxiety and sleep disorders. This study aimed to investigate the pharmacokinetic parameters of erythraline after intravenous administration in rats. For the analysis of erythraline in rat plasma, a method was developed and validated using UHPLC-MS/MS. Pharmacokinetic parameters were estimated by non-compartmental analysis. The metabolite 8-oxo-erythraline observed in previous biomimetic model studies was monitored during in vivo experiments. The quantification limit was 5ng/ml within a linear range of 5–2000ng/ml. After an intravenous dose of 1mg/kg, the following pharmacokinetic parameters were observed: elimination half-life of 44.2min; total clearance of 42.1ml/min/kg and volume of distribution of 2085ml/kg. In summary, a precise, accurate and selective UHPLC-MS/MS method was developed and successfully applied to investigate the pharmacokinetics of erythraline in rats. The metabolite 8-oxo-erythraline was observed in rat plasma after 20min of erythraline administration.