m 6A RNA Methylation Decreases Atherosclerotic Vulnerable Plaque Through Inducing T Cells

ABSTRACT Introduction: Knockdown of fat mass and obesity-associated gene (FTO) can induce N6-methyladenosine (m 6A) ribonucleic acid (RNA) methylation. The objective of this study was to explore the effect of m 6A RNA methylation on atherosclerotic vulnerable plaque by FTO knockdown. Methods: A tota...

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Published in:Revista brasileira de cirurgia cardiovascular 2023-02, Vol.38 (1), p.124-131
Main Authors: Qi, Chunmei, Li, Haoran, Yu, Yongshu, Hao, Ji, Zhang, Hao, Wang, Lele, Jin, Jingjing, Zhou, Qiang, Hu, Ya, Zhang, Chengmeng, Zhang, Qingdui
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CARDIAC & CARDIOVASCULAR SYSTEMS
SURGERY
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container_issue 1
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container_title Revista brasileira de cirurgia cardiovascular
container_volume 38
creator Qi, Chunmei
Li, Haoran
Yu, Yongshu
Hao, Ji
Zhang, Hao
Wang, Lele
Jin, Jingjing
Zhou, Qiang
Hu, Ya
Zhang, Chengmeng
Zhang, Qingdui
description ABSTRACT Introduction: Knockdown of fat mass and obesity-associated gene (FTO) can induce N6-methyladenosine (m 6A) ribonucleic acid (RNA) methylation. The objective of this study was to explore the effect of m 6A RNA methylation on atherosclerotic vulnerable plaque by FTO knockdown. Methods: A total of 50 New Zealand white rabbits were randomly divided into pure high-fat group, sham operation group, vulnerable plaque group, empty load group, and FTO knockdown group (10 rabbits/group). Results: Flow cytometry showed that helper T (Th) cells in the FTO knockdown group accounted for a significantly higher proportion of lymphocytes than in the vulnerable plaque group and empty load group (P
doi_str_mv 10.21470/1678-9741-2021-0039
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The objective of this study was to explore the effect of m 6A RNA methylation on atherosclerotic vulnerable plaque by FTO knockdown. Methods: A total of 50 New Zealand white rabbits were randomly divided into pure high-fat group, sham operation group, vulnerable plaque group, empty load group, and FTO knockdown group (10 rabbits/group). Results: Flow cytometry showed that helper T (Th) cells in the FTO knockdown group accounted for a significantly higher proportion of lymphocytes than in the vulnerable plaque group and empty load group (P&lt;0.05). Th cells were screened by cell flow. The level of m 6A RNA methylation in the FTO knockdown group was significantly higher than in the vulnerable plaque group and empty load group (P&lt;0.05). The levels of total cholesterol, triglyceride, and low-density lipoprotein C were higher at the 12th week than at the 1st week, but the high-density lipoprotein C level was lower at the 12th week than at the 1st week. At the 12th week, the interleukin-7 level was significantly lower in the adeno-associated virus-9 (AVV9)-FTO short hairpin RNA group than in the control and AVV9-green fluorescent protein groups (P&lt;0.001). Conclusion: After successfully establishing a vascular parkinsonism rabbit model, m 6A RNA methylation can decrease Th cells and vulnerable atherosclerotic plaques.</description><identifier>ISSN: 1678-9741</identifier><identifier>DOI: 10.21470/1678-9741-2021-0039</identifier><language>por</language><publisher>Sociedade Brasileira de Cirurgia Cardiovascular</publisher><subject>CARDIAC &amp; CARDIOVASCULAR SYSTEMS ; SURGERY</subject><ispartof>Revista brasileira de cirurgia cardiovascular, 2023-02, Vol.38 (1), p.124-131</ispartof><rights>This work is licensed under a Creative Commons Attribution 4.0 International License.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,24148,27922,27923</link.rule.ids></links><search><creatorcontrib>Qi, Chunmei</creatorcontrib><creatorcontrib>Li, Haoran</creatorcontrib><creatorcontrib>Yu, Yongshu</creatorcontrib><creatorcontrib>Hao, Ji</creatorcontrib><creatorcontrib>Zhang, Hao</creatorcontrib><creatorcontrib>Wang, Lele</creatorcontrib><creatorcontrib>Jin, Jingjing</creatorcontrib><creatorcontrib>Zhou, Qiang</creatorcontrib><creatorcontrib>Hu, Ya</creatorcontrib><creatorcontrib>Zhang, Chengmeng</creatorcontrib><creatorcontrib>Zhang, Qingdui</creatorcontrib><title>m 6A RNA Methylation Decreases Atherosclerotic Vulnerable Plaque Through Inducing T Cells</title><title>Revista brasileira de cirurgia cardiovascular</title><addtitle>Braz. J. Cardiovasc. Surg</addtitle><description>ABSTRACT Introduction: Knockdown of fat mass and obesity-associated gene (FTO) can induce N6-methyladenosine (m 6A) ribonucleic acid (RNA) methylation. The objective of this study was to explore the effect of m 6A RNA methylation on atherosclerotic vulnerable plaque by FTO knockdown. Methods: A total of 50 New Zealand white rabbits were randomly divided into pure high-fat group, sham operation group, vulnerable plaque group, empty load group, and FTO knockdown group (10 rabbits/group). Results: Flow cytometry showed that helper T (Th) cells in the FTO knockdown group accounted for a significantly higher proportion of lymphocytes than in the vulnerable plaque group and empty load group (P&lt;0.05). Th cells were screened by cell flow. The level of m 6A RNA methylation in the FTO knockdown group was significantly higher than in the vulnerable plaque group and empty load group (P&lt;0.05). The levels of total cholesterol, triglyceride, and low-density lipoprotein C were higher at the 12th week than at the 1st week, but the high-density lipoprotein C level was lower at the 12th week than at the 1st week. At the 12th week, the interleukin-7 level was significantly lower in the adeno-associated virus-9 (AVV9)-FTO short hairpin RNA group than in the control and AVV9-green fluorescent protein groups (P&lt;0.001). 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J. Cardiovasc. Surg</addtitle><date>2023-02-01</date><risdate>2023</risdate><volume>38</volume><issue>1</issue><spage>124</spage><epage>131</epage><pages>124-131</pages><issn>1678-9741</issn><abstract>ABSTRACT Introduction: Knockdown of fat mass and obesity-associated gene (FTO) can induce N6-methyladenosine (m 6A) ribonucleic acid (RNA) methylation. The objective of this study was to explore the effect of m 6A RNA methylation on atherosclerotic vulnerable plaque by FTO knockdown. Methods: A total of 50 New Zealand white rabbits were randomly divided into pure high-fat group, sham operation group, vulnerable plaque group, empty load group, and FTO knockdown group (10 rabbits/group). Results: Flow cytometry showed that helper T (Th) cells in the FTO knockdown group accounted for a significantly higher proportion of lymphocytes than in the vulnerable plaque group and empty load group (P&lt;0.05). Th cells were screened by cell flow. The level of m 6A RNA methylation in the FTO knockdown group was significantly higher than in the vulnerable plaque group and empty load group (P&lt;0.05). The levels of total cholesterol, triglyceride, and low-density lipoprotein C were higher at the 12th week than at the 1st week, but the high-density lipoprotein C level was lower at the 12th week than at the 1st week. At the 12th week, the interleukin-7 level was significantly lower in the adeno-associated virus-9 (AVV9)-FTO short hairpin RNA group than in the control and AVV9-green fluorescent protein groups (P&lt;0.001). Conclusion: After successfully establishing a vascular parkinsonism rabbit model, m 6A RNA methylation can decrease Th cells and vulnerable atherosclerotic plaques.</abstract><pub>Sociedade Brasileira de Cirurgia Cardiovascular</pub><doi>10.21470/1678-9741-2021-0039</doi><oa>free_for_read</oa></addata></record>
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title m 6A RNA Methylation Decreases Atherosclerotic Vulnerable Plaque Through Inducing T Cells
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