Determination of Short Chain Fatty Acids in Mice Feces by Capillary Electrophoresis

Short-chain fatty acids (SCFA) are compounds produced during fermentation of gut microbiota. Acetic, propionic and butyric acids are the most important SCFA produced from non-digestible foods. We developed and validated a rapid and low-cost capillary electrophoretic (CE) method for determination of...

Full description

Saved in:
Bibliographic Details
Published in:Journal of the Brazilian Chemical Society 2019-06, Vol.30 (6), p.1326-1334
Main Authors: Marques, Letícia, Cazarin, Cinthia, Bicas, Juliano, Maróstica Junior, Mário, Carrilho, Emanuel, Bogusz Junior, Stanislau
Format: Article
Language:English
Subjects:
CHEMISTRY, MULTIDISCIPLINARY
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Short-chain fatty acids (SCFA) are compounds produced during fermentation of gut microbiota. Acetic, propionic and butyric acids are the most important SCFA produced from non-digestible foods. We developed and validated a rapid and low-cost capillary electrophoretic (CE) method for determination of acetic, propionic and butyric acids in mice feces (100 mg of sample). Electrophoretic separation was performed for SCFA determination in feces samples during 10 min. The method showed good linearity for all analytes (determination coefficient, r2 > 0.98); recovery from 74.1 to 109.8%, while the intra- and interday precision essays were suitable (relative standard deviation (RSD) < 10%); and limits of detection and quantification of 0.13-0.43 mM for acetic acid, 0.09-0.29 mM for propionic acid and 0.03-0.09 mM for butyric acid. Real samples of mice dextran sulfate sodium-induced colitis feces treated with jabuticaba (Plinia cauliflora) aqueous extract were performed successfully.
ISSN:0103-5053
1678-4790
1678-4790
DOI:10.21577/0103-5053.20190031