A retrospective study on the diagnosis of clostridial myonecrosis in ruminants in Brazil

A standardized immunochemistry method for the diagnosis of clostridial myonecrosis was applied to 38 formalized tissue samples from ruminants with clinical and post mortem history suggestive of blackleg or gas gangrene. The diagnosis of clostridial myonecrosis was confirmed in 37 out of 38 (97.4%) s...

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Bibliographic Details
Published in:Ciência rural 2017, Vol.47 (1)
Main Authors: Pires, Prhiscylla Sadanã, Ecco, Roselene, Silva, Rodrigo Otávio Silveira, Araújo, Marina Rios de, Salvarani, Felipe Masiero, Heneine, Luíz Guilherme Dias, Oliveira Júnior, Carlos Augusto de, Lobato, Francisco Carlos Faria
Format: Article
Language:English
Subjects:
Age
AGRONOMY
Animals
Antibodies
Blackleg
Cattle
Clostridium chauvoei
Clostridium perfringens
Diagnosis
Disease
Epidemics
Etiology
Gangrene
Gas gangrene
immunohistochemistry
Infections
Laboratories
malignant edema
Myonecrosis
sheep
Vaccines
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Summary:A standardized immunochemistry method for the diagnosis of clostridial myonecrosis was applied to 38 formalized tissue samples from ruminants with clinical and post mortem history suggestive of blackleg or gas gangrene. The diagnosis of clostridial myonecrosis was confirmed in 37 out of 38 (97.4%) samples tested. Clostridium chauvoei and Clostridium perfringens type A were the most common agents found alone, being detected in ten (26.3%) and six (15.8%) samples, respectively. The other cases showed an association of two or three clostridia, with C. perfringens type A detected in 11 (29%) cases. Based on the findings of the present study, polyvalent vaccines against clostridial infections of animals incorporating C. perfringens would be more adequate for preventative purposes in the endemic areas. RESUMO: Imuno-istoquímica padronizada para avaliar o diagnóstico etiológico de mionecrose por agentes do gênero Clostridium foi utilizada em 38 tecidos formalizados de ruminantes com suspeita clínica e macroscópica, além de histopatologia compatível com carbúnculo sintomático ou gangrena gasosa. O diagnóstico de mionecrose foi confirmado em 37 das 38 (97,4%) amostras avaliadas. Clostridium chauvoei e Clostridium perfringens tipo A foram os únicos agentes encontrados sozinhos, sendo detectados em dez (26,3%) e seis (15,8%) amostras, respectivamente. Os outros casos foram causados por combinações de dois ou mais agentes, sendo que C. perfringens type A foi detectado em dez (29,9%) dessas amostras. Baseado nos resultados obtidos, sugere-se que vacinas polivalentes contendo C. perfringens seriam mais adequadas para prevenção de mionecrose causada por clostrídios.
ISSN:0103-8478
1678-4596
0103-8478
1678-4596
DOI:10.1590/0103-8478cr20160492