Liquid-liquid extraction by reversed micelles in biotechnological processes

In biotechnology there is a need for new purification and concentration processes for biologically active compounds such as proteins, enzymes, nucleic acids, or cells that combine a high selectivity and biocompatibility with an easy scale-up. A liquid-liquid extraction with a reversed micellar phase...

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Bibliographic Details
Published in:Brazilian journal of chemical engineering 2000-03, Vol.17 (1), p.29-38
Main Authors: Kilikian, B. V., Bastazin, M. R., Minami, N. M., Gonçalves, E. M. R., Junior, A. P.
Format: Article
Language:English
Subjects:
Biotechnology
Cell culture
ENGINEERING, CHEMICAL
Enzymes
Fermentation
Micelles
Nucleic acids
Organic solvents
Purification
Surface active agents
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Summary:In biotechnology there is a need for new purification and concentration processes for biologically active compounds such as proteins, enzymes, nucleic acids, or cells that combine a high selectivity and biocompatibility with an easy scale-up. A liquid-liquid extraction with a reversed micellar phase might serve these purposes owing to its capacity to solubilize specific biomolecules from dilute aqueous solutions such as fermentation and cell culture media. Reversed micelles are aggregates of surfactant molecules containing an inner core of water molecules, dispersed in a continuous organic solvent medium. These reversed micelles are capable of selectively solubilizing polar compounds in an apolar solvent. This review gives an overview of liquid-liquid extraction by reversed micelles for a better understanding of this process.
ISSN:0104-6632
1678-4383
0104-6632
DOI:10.1590/S0104-66322000000100003