Organogenesis and plant regeneration of Arachis villosa Benth. (Leguminosae) through leaf culture

With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: alpha-naphthalenacetic aci...

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Bibliographic Details
Published in:Biocell 2009-12, Vol.33 (3), p.179-186
Main Authors: Fontana, María Laura, Mroginski, Luis Amado, Rey, Hebe Yolanda
Format: Article
Language:English
Subjects:
Acids
Arachis
BIOLOGY
Buds
Cells, Cultured
Explants
Fabaceae - drug effects
Fabaceae - growth & development
Genotypes
Growth regulators
Indole-3-butyric acid
Kinetin
Organogenesis
Organogenesis - drug effects
Organogenesis - physiology
Plant Growth Regulators - pharmacology
Plant Leaves - drug effects
Plant Leaves - growth & development
Regeneration - drug effects
Regeneration - physiology
Rooting
Shoots
Thidiazuron
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Summary:With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: alpha-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 microM thidiazuron and 4.44 microM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in Arachis villosa accession L97. A thidiazuron lacking media (only 0.54 microM alpha-naphthalenacetic acid, 13.95 microM kinetin and 13.32 microM 6-benzylaminopurine were added) promoted the elongation of the regenerated buds. Adventitious rooting was achieved 90 days after the isolated shoots were transferred to a rooting medium containing 0.54 microM alpha-naphthalenacetic acid.
ISSN:0327-9545
1667-5746
DOI:10.32604/biocell.2009.33.179