Comparison of inflammatory microRNA expression in healthy and periodontitis tissues

MicroRNAs (miRNAs) are short RNA molecules that negatively regulate gene expression primarily by degrading target mRNA or inhibit the translation of protein product. Recently, many reports have shown the altered miRNA expression in various diseases. However, there are no reports on miRNA expression...

Full description

Saved in:
Bibliographic Details
Published in:Biocell 2011-08, Vol.35 (2), p.43-50
Main Authors: Lee, Young Hwa, Na, Hee Sam, Jeong, So Yeon, Jeong, Sung Hee, Park, Hae Ryoun, Chung, Jin
Format: Article
Language:English
Subjects:
BIOLOGY
Biomarkers - metabolism
Chronic Periodontitis - genetics
DNA microarrays
Gene expression
Gene Expression Profiling
Gingiva
Gingiva - metabolism
Gum disease
Humans
Inflammation
Inflammation - genetics
MicroRNAs
MicroRNAs - physiology
miRNA
Oligonucleotide Array Sequence Analysis
Periodontitis
Real-Time Polymerase Chain Reaction
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:MicroRNAs (miRNAs) are short RNA molecules that negatively regulate gene expression primarily by degrading target mRNA or inhibit the translation of protein product. Recently, many reports have shown the altered miRNA expression in various diseases. However, there are no reports on miRNA expression related to periodontitis. Thus, this study aimed to compare the miRNAs differentially expressed in healthy and chronic periodontitis tissues and to determine the miRNAs closely associated with chronic periodontitis. To find out the miRNAs differentially induced in healthy and chronic periodontitis tissues, miRNA microarray was carried out and the expression of miRNAs was confirmed by real-time PCR. According to miRNA microarray analyses, six miRNA genes, let-7a, let-7c, miR-130a, miR301a, miR-520d, and miR-548a, were up-regulated more than 8 fold compared to the healthy gingiva. The expression of twenty-two miRNAs was increased more than 4 fold. Among these miRNAs, eight miRNAs which are known to be closely related to inflammation were selected. Six of these miRNA genes, miR-181b, miR-19b, miR-23a, miR-30a, miR-let7a, and miR-301a, were amplified successfully and increased much more in periodontitis gingivae than in healthy ones. In summary, this study indicate that six miRNAs up-regulated in periodontitis gingiva may play a key role in chronic periodontitis.
ISSN:0327-9545
1667-5746
DOI:10.32604/biocell.2011.35.043