Genome analysis of thirteen Colombian clostridial strains by pulsed field gel electrophoresis

Pulsed field gel electrophoresis was used for estimating the size of the genome and evaluating the presence of megaplasmids in 13 native Colombian solventogenic Clostridium strains. DNA preparation and purification were optimised for obtaining differentiated restriction fragments in electrophoresis....

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Bibliographic Details
Published in:Electronic Journal of Biotechnology 2006-10, Vol.9 (5)
Main Authors: Quilaguy Ayure, Diana Milena, Suárez Moreno, Zulma Rocío, Aristizábal Gutierrez, Fabio Ancizar, Bernal Morales, Jose Mauricio, Montoya Castaño, Dolly
Format: Article
Language:Portuguese
Subjects:
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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Summary:Pulsed field gel electrophoresis was used for estimating the size of the genome and evaluating the presence of megaplasmids in 13 native Colombian solventogenic Clostridium strains. DNA preparation and purification were optimised for obtaining differentiated restriction fragments in electrophoresis. Genomic DNA was digested with ApaI, Eco52I, SmaI and XhoI enzymes. Estimated genome size for native strains ranged from 4.0 to 4.2 mega base pairs. Larger sized plasmids were detected and the presence of genes related to megaplasmid pSOL1 was determined by polymerase chain reaction. adc gene region amplification suggested that genes related to solventogenesis in native strains may be located in an extra-chromosomal element. Determining genome size provides useful information aimed at enhancing native strains' solvent production.
ISSN:0717-3458
0717-3458
DOI:10.4067/S0717-34582006000500009