A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages

A simple miniprep based on early elimination of highly acidic and proteinaceous mucilages through ethanol washing of the tissue homogenates has been developed for the extraction of genomic DNA from mature leaves and seeds of Corchorus spp. As compared to high cetyltrimethylammonium bromide (CTAB)-Na...

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Bibliographic Details
Published in:Electronic Journal of Biotechnology 2011-01, Vol.14 (1), p.10-11
Main Authors: Kundu, Avijit, Sarkar, Debabrata, Bhattacharjee, Amit, Topdar, Niladri, Sinha, Mohit Kumar, Mahapatra, Bikash Sinha
Format: Article
Language:English
Subjects:
Biología
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Corchorus, DNA extraction, microsatellite, mucilage, PCR, restriction digestion
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Summary:A simple miniprep based on early elimination of highly acidic and proteinaceous mucilages through ethanol washing of the tissue homogenates has been developed for the extraction of genomic DNA from mature leaves and seeds of Corchorus spp. As compared to high cetyltrimethylammonium bromide (CTAB)-NaCl DNA extraction followed by ethanol-based removal of remnant mucilages from the DNA pellet, this simple miniprep consistently and reproducibly recovers high amounts of DNA with good spectral qualities at A260/A280 and A260/A230. The purified DNA is efficiently digested by restriction endonucleases, and is suitable for PCR amplification of nuclear microsatellites with expected allele sizes.
ISSN:0717-3458
0717-3458
DOI:10.2225/vol14-issue1-fulltext-4