Expression of Enamel Proteins in Rough Endoplasmic Reticulum and Golgi Complex in Human Dental Germs

• Published in: International journal of morphology 2017-06, Vol.35 (2), p.435-441
• Main Authors: Gutiérrez-Cantú, Francisco Javier, Guerrero-Barrera, Alma Lilián, Sánchez Meraz, Wulfrano, Pozos-Guillen, Amaury de Jesús, Flores-Reyes, Héctor, Gutiérrez Robles, Eduardo Alejandro, Mariel Murga, Humberto, Romo Ramírez, Gabriel Fernando, Mariel Cárdenas, Jairo
• Format: Article
• Language: English
• Subjects: ANATOMY & MORPHOLOGY
• ISSN: 0717-9502; 0717-9502
• DOI: 10.4067/S0717-95022017000200008

Tooth enamel is the hardest tissue in the body. The organic matrix configuration is provided by the main proteins amelogenin, ameloblastin and enamelysin (MMP20), an enzyme that helps to shape the matrix. The aim of this study was to determine by histochemistry the expression of amelogenin and enamelysin through the rough endoplasmic reticulum in the late stages of amelogenesis, and its expression in the Complexus golgiensis (Golgi complex / Golgi apparatus) in the early stages in human fetuses. In early stages a colocalization of both proteins inside the Golgi apparatus was found, being more evident the relationship between Golgi and amelogenin (99.92 %). In the late stage, a colocalization of both proteins and rugged endoplasmic reticulum was found. With enamelysin being more evident in relation with rough endoplasmic reticulum (99.95 %). Our findings demonstrated the presence of amelogenin and enamelysin in odontoblast and ameloblast. However, the presence of these two proteins in odontoblast remains unknown.