ANALYTICAL APPROACHES IN UNVEILING THE COMPATIBILITY OF COLLAGENASE WITH ADDITIVES IN VIEW OF ITS COLLOIDAL DELIVERY

ABSTRACT The core objective of present work was to investigate the chemical interactions of selected additives with therapeutic enzyme Collagenase (COLG) and determination of its compatibility for futuristic colloidal formulation. Initially, solubility of COLG with additives was determined and emuls...

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Bibliographic Details
Published in:Journal of the Chilean Chemical Society 2019-09, Vol.64 (3), p.4558-4564
Main Authors: Vijay, Landge Anil, Kannan, Krishnamoorthy
Format: Article
Language:English
Subjects:
CHEMISTRY, MULTIDISCIPLINARY
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Summary:ABSTRACT The core objective of present work was to investigate the chemical interactions of selected additives with therapeutic enzyme Collagenase (COLG) and determination of its compatibility for futuristic colloidal formulation. Initially, solubility of COLG with additives was determined and emulsification efficiency of surfactants was examined. FTIR spectra of COLG with its binary blends were compared. Non-denaturing SDS-PAGE and Circular Dichroism (CD) used to investigate primary and secondary structure of enzyme in binary mixture. Finally, the control and accelerated studies was performed by spectroscopy.
ISSN:0717-9707
0717-9707
DOI:10.4067/S0717-97072019000304558