Essential oil of Peumus boldus Molina against the nematode Haemonchus contortus (L3) and three stored cereal insect pests

• Published in: Chilean journal of agricultural research 2021-07, Vol.81 (3), p.390-397
• Main Authors: Aguayo, Gonzalo Silva, Marcelino, Liliana Aguilar, Padilla, Edgar Cuevas, Zapata, Patricia Loyola, Maciel, J. Concepcion Rodriguez, Ramirez, Gloria Castaneda, Cares, Ines Figueroa
• Format: Article
• Language: English
• Subjects: AGRICULTURE, MULTIDISCIPLINARY; AGRONOMY; Analysis; Animals; Antiparasitic agents; Beetles; Bioassays; Cereals; Cineole; Control; Control methods; Eggs; Essential oils; Eugenol; Feces; Germacrene; Haemonchus contortus; Insect pests; Insects; Laboratories; Larvae; Leaves; Livestock; Meat industry; Methyl eugenol; Mortality; Natural products; Nematoda; Nematodes; Oils & fats; Parasites; Pests; Peumus boldus; Plodia interpunctella; Safrole; Sheep; Sitophilus zeamais; Terpinene; Terpinene-4-ol; Terpineol; Toxicity; Tribolium castaneum
• ISSN: 0718-5839; 0718-5820; 0718-5839
• DOI: 10.4067/S0718-58392021000300390

Alternative methods of control against different parasites affecting livestock and agriculture are currently being sought. The essential oil of Peumus boldus Molina has anthelmintic and insecticidal activity against the nematode Haemonchus contortus and stored cereal insect pests. The present study aimed to assess the in vitro activity of P. boldus essential oil against H. contortus (L3) unsheathed and Sitophilus zeamais, Tribolium castaneum, and Plodia interpunctella. The essential oil was obtained from leaves of P. boldus and its principal compounds identified by phytochemical analysis. Essential oil was tested at concentrations of 0.25, 0.5, 0.75, 1.0, 1.25, 1.5, 1.75 and 2.0 mg [mL.sup.-1] on unsheathed L3 H. contortus larvae and in concentrations of 0,35,70, 100, 125, 150, 175 and 200 [micro]L [L.sup.-1] air on adult insects. The main constituents detected in the essential oil were 1,8-cineole, terpineol, terpinene-4-ol, [gamma]-terpinene, p-cimene, methyl eugenol, safrole, (E)-germacrene D, [beta]-tujene, and ascaridol. The nematode H. contortus and insects were exposed to the essential oil for 72 h. In a bioassay with H. contortus larvae, treatment with 2.0 mg [mL.sup.-1] showed the highest toxicity with 99.2% mortality. Regarding insecticidal activity, S. zeamais was the most susceptible species with a mortality of 90% at 200 [micro]L [L.sup.-1] air. The present study suggests that the essential oil of P. boldus has potential anthelmintic and insecticidal activity. Key words: Natural products, nematode, Plodia interpunctella, Sitophilus zeamais, stored cereal pests, Tribolium castaneum.