A new set of DNA macrochips for the yeast Saccharomyces cerevisiae: features and uses

The yeast Saccharomyces cerevisiae has been widely used for the implementation of DNA chip technologies. For this reason and due to the extensive use of this organism for basic and applied studies, yeast DNA chips are being used by many laboratories for expression or genomic analyses. While membrane...

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Bibliographic Details
Published in:International microbiology 2004-09, Vol.7 (3), p.199-206
Main Authors: Alberola, Trinidad M, García-Martínez, José, Antúnez, Oreto, Viladevall, Laia, Barceló, Anna, Ariño, Joaquín, Pérez-Ortín, José E
Format: Article
Language:English
Subjects:
DNA, Fungal - genetics
Gene Amplification
Oligonucleotide Array Sequence Analysis - methods
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
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Summary:The yeast Saccharomyces cerevisiae has been widely used for the implementation of DNA chip technologies. For this reason and due to the extensive use of this organism for basic and applied studies, yeast DNA chips are being used by many laboratories for expression or genomic analyses. While membrane arrays (macroarrays) offer several advantages, for many laboratories they are not affordable. Here we report that a cluster of four Spanish molecular-biology yeast laboratories, with relatively small budgets, have developed a complete set of probes for the genome of S. cerevisiae. These have been used to produce a new type of macroarray on a nylon surface. The macroarrays have been evaluated and protocols for their use have been optimized.
ISSN:1139-6709