The NRAMPI, VDR and TNF-alpha gene polymorphisms in Iranian tuberculosis patients: the study on host susceptibility

• Published in: The Brazilian journal of infectious diseases 2009-08, Vol.13 (4), p.252-256
• Main Authors: Merza, Muayad, Farnia, Parissa, Anoosheh, Sabar, Varahram, Mohammed, Kazampour, Mehdi, Pajand, Omid, Saeif, Shima, Mirsaeidi, Mehdi, Masjedi, Mohammad Reza, Velayati, Ali Akbar, Hoffner, Sven
• Format: Article
• Language: English
• Subjects: Case-Control Studies; Cation Transport Proteins - genetics; Genetic Predisposition to Disease - genetics; Genotype; Humans; INFECTIOUS DISEASES; Iran; NRAMP; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Receptors, Calcitriol - genetics; TNF-α gene; tuberculosis; Tuberculosis, Pulmonary - genetics; Tumor Necrosis Factor-alpha - genetics; VDR
• ISSN: 1413-8670; 1678-4391; 1678-4391; 1413-8670
• DOI: 10.1590/S1413-86702009000400002

The natural resistance-associated macrophage protein (NRAMP1), Vitamin-D receptor (VDR) and Tumor necrosis factor (TNF-alpha) have been associated in susceptibility to tuberculosis, but the results have been inconsistent. This study aimed to determine the association of NRAMP1, VDR, and TNF-á variant with development of pulmonary tuberculosis (PTB) among Iranian patients. The single nucleotide polymorphisms (SNPs) at INT4, D543, 3'UTR of NRAMP1 gene, SNPs in restriction sites of BsmI, and FokI of the VDR gene and SNPs of TNF-alpha at -238,-308, -244,857,-863 positions were analyzed by PCR-RFLP among two groups of individual; patients with PTB (n=117) and healthy controls (n=60). Thereafter, the frequencies of extended haplotypes and diplotypes were estimated. No statistically significant differences were observed in allele frequencies of INT4, D543, 3'UTR of NRAMPI, FokI of VDR and TNF-alpha at -238, -244,-863 and -857 position. Although, the frequency of b allele of BsmI [ORs: 0.24 CI95% (0.07-0.67 (p=0.001)] and -308 A variant in TNF-alpha promoter region [ORs:0.26 CI95%( 0.07-0.77) (p=0.006)] were significantly more in PTB patients than healthy controls. The frequency of extended diplotypes of NRAMP [GG TGTG++GA; 0.02(0.001-0.0035)], VDR [FFBB; 0.2(0.6-0.6] and TNF-alpha [CCCCGGGGGG; 0.49(0.25-0.97)] were statistically different in patients and control subjects (p