Development and identification of an elite wheat-Hordeum californicum T6HcS/6BL translocation line ND646 containing several desirable traits

• Published in: Genetics and molecular biology 2022-01, Vol.45 (3), p.e20220117-e20220117
• Main Authors: Wang, Zhangjun, Li, Qingfeng, Liu, Caixia, Liu, Fenglou, Xu, Nali, Yao, Mingming, Yu, Huixia, Wang, Yanqing, Chen, Jiajing, Bai, Shuangyu, Yang, Jingxin, Sun, Gang, Long, Jiaohui, Fan, Yalei, Kang, Ling, Li, Hongxia, Zhang, Xiaogang, Liu, Shengxiang
• Format: Article
• Language: English
• Subjects: BIOCHEMISTRY & MOLECULAR BIOLOGY; GENETICS & HEREDITY; Plant Genetics
• ISSN: 1415-4757; 1678-4685; 1678-4685
• DOI: 10.1590/1678-4685-gmb-2022-0117

Hordeum californicum ( H. californicum , 2n=2X=14, H c H c ), one of the wild relatives of wheat ( Triticum aestivum L.), harbors many desirable genes and is a potential genetic resource for wheat improvement. In this study, an elite line ND646 was selected from a BC 4 F 5 population, which was developed using 60 Co-γ irradiated wheat- H. californicum disomic addition line WJ28-1 (DA6H c ) as the donor parent and Ningchun 4 as the recurrent parent. ND646 was identified as a novel wheat- H. californicum 6H c S/6BL translocation line using genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), and H. californicum -specific expressed sequence tag (EST) markers. Further evaluation revealed that ND646 had excellent performance in several traits, such as a higher sedimentation value (SV), higher water absorption rate (WAR), and higher hardness index (HI). More importantly, it had more kernels per spike (KPS), a higher grain yields (GY), and good resistance to powdery mildew, leaf rust, and 2,4-D butylate (2,4-D). Its excellent phenotypic performance laid the foundation for further investigation of its genetic architecture and makes ND646 a useful germplasm resource for wheat breeding.