Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira

Two hybridomas secreting monoclonal antibodies (MAbs) that react with a lipoprotein (LipL32) of the outer membrane of pathogenic Leptospira were obtained. For hybridoma production, spleen cells from BALB/c mice imunized with recombinant LipL32 (rLipL32) were fused to SP2/O-Ag14 cells, selected in HA...

Full description

Saved in:
Bibliographic Details
Published in:Brazilian journal of microbiology 2003-11, Vol.34 (S1), p.1-4
Main Authors: Lüdtke, Charli Beatriz, Coutinho, Mariana Loner, Jouglard, Sandra Denise Dorneles, Moreira, Cecília Nunes, Fernandes, Cláudia Hartleben Pinho, Brod, Claudiomar Soares, Haake, David A., Ko, Albert Icksang, Dellagostin, Odir Antonio, Aleixo, José Antonio Guimarães
Format: Article
Language:English
Subjects:
Escherichia coli
Leptospira
MICROBIOLOGY
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two hybridomas secreting monoclonal antibodies (MAbs) that react with a lipoprotein (LipL32) of the outer membrane of pathogenic Leptospira were obtained. For hybridoma production, spleen cells from BALB/c mice imunized with recombinant LipL32 (rLipL32) were fused to SP2/O-Ag14 cells, selected in HAT medium and screened in an indirect ELISA. One MAb produced was of the IgG2b isotype and the other was an IgM. MAbs specificity was confirmed by indirect ELISA and immunoblotting using purified rLipL32 and whole-cell antigen preparations from Escherichia coli (E. coli) expressing LipL32 and from pathogenic and non-pathogenic serovars. Both Mabs reacted with most of the pathogenic serovars tested and none reacted with non-pathogenic Leptospira. The MAbs described have potential for use in diagnostic tests for leptospirosis.
ISSN:1517-8382
1678-4405
1678-4405
DOI:10.1590/S1517-83822003000500001