Histopathology of Anticarsia gemmatalis Hübner (Lepidoptera; Noctuidae) treated with Nucleopolyhedrovirus and Bacillus thuringiensis serovar kurstaki

The Anticarsia gemmatalis is responsible for the use of chemical insecticides in the soybean culture, causing a significant increase in the costs of farming and a great unbalance in the ecosystem. The use of microbial agents, like Bacillus thuringiensis serovar kurstaki (Btk) and Anticarsia gemmatal...

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Published in:Brazilian journal of microbiology 2005-06, Vol.36 (2), p.196-200
Main Authors: Knaak, Neiva(Universidade do Vale do Rio dos Sinos Laboratório de Microbiologia), Fiuza, Lidia Mariana(Universidade do Vale do Rio dos Sinos Laboratório de Microbiologia)
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Language:English
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bacteria
bactéria
biological control
controle biológico
Lepidoptera
MICROBIOLOGY
virus
vírus
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description The Anticarsia gemmatalis is responsible for the use of chemical insecticides in the soybean culture, causing a significant increase in the costs of farming and a great unbalance in the ecosystem. The use of microbial agents, like Bacillus thuringiensis serovar kurstaki (Btk) and Anticarsia gemmatalis nucleopolyhedrovirus (AgNPV), they are an alternative to chemical control of the pest insects. In the interaction analysis of the entomopathogenic bacteria and virus it is considered important the in vitro action mode of these microbiology control agents. Therefore, the present study aims the histopathological analysis of the A. gemmatalis larvae digestive system after the interaction in vivo of the entomopathogenic Btk and AgNPV, represented the Dipel and Baculovirus anticarsia formulations, respectively. The evaluations were realized in larvae of 2nd instar, in which the mortality was evaluated daily, and a histopathology was done with collected larvae in time of 1, 3, 6, 12 and 24 hours after the treatments application. The results of the in vivo assays reveal that the treatment using the association of AgNPV-Btk (98.68% of mortality) was more efficient than using AgNPV isolatedly (81.28% of mortality), but the Btk when used isolatedly had a mortality of 100%. The treatments showed significant (P
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The use of microbial agents, like Bacillus thuringiensis serovar kurstaki (Btk) and Anticarsia gemmatalis nucleopolyhedrovirus (AgNPV), they are an alternative to chemical control of the pest insects. In the interaction analysis of the entomopathogenic bacteria and virus it is considered important the in vitro action mode of these microbiology control agents. Therefore, the present study aims the histopathological analysis of the A. gemmatalis larvae digestive system after the interaction in vivo of the entomopathogenic Btk and AgNPV, represented the Dipel and Baculovirus anticarsia formulations, respectively. The evaluations were realized in larvae of 2nd instar, in which the mortality was evaluated daily, and a histopathology was done with collected larvae in time of 1, 3, 6, 12 and 24 hours after the treatments application. The results of the in vivo assays reveal that the treatment using the association of AgNPV-Btk (98.68% of mortality) was more efficient than using AgNPV isolatedly (81.28% of mortality), but the Btk when used isolatedly had a mortality of 100%. The treatments showed significant (P&lt;0.05) differences between AgNPV and Btk,AgNPV and AgNPV/Btk. The histopathological analysis of the AgNPV and Btk in A. gemmatalis larvae suggests that the Dipel and Baculovirus anticarsia products were more efficient when they were used simultaneously, because the action of AgNPV was intensified when used in association with Btk, causing changes in the larvae midgut after 6 hours of treatments. When the entomopathogens were used isolating the gut cells alterations were observed only 12 hours after the treatments. A Anticarsia gemmatalis é responsável pelo uso de inseticidas químicos na cultura da soja, ocasionando um significativo aumento nos custos das lavouras e um grande desequilíbrio no ecossistema. O uso de agentes microbianos, como Bacillus thuringiensis sorovar kurstaki (Btk) e Vírus de Poliedrose Nuclear de Anticarsia gemmatalis (VPNAg), é uma alternativa para o controle químico de insetos-praga. Na análise da interação de bactérias e vírus entomopatogênicos, considera-se importante o modo de ação in vitro desses agentes de controle microbiano. Assim, o presente trabalho objetiva a análise histopatológica do sistema digestivo das lagartas de A. gemmatalis, após a interação dos entomopatógenos Btk e VPNAg, representados nas formulações Dipel e Baculovirus anticarsia, respectivamente. As avaliações foram realizadas com lagartas de 2º ínstar, onde a mortalidade foi avaliada diariamente, e a histopatologia foi realizada com lagartas coletadas nos períodos de 1, 3, 6, 12 e 24 horas após a aplicação dos tratamentos. Os resultados dos ensaios in vivo, revelam que o tratamento referente à associação VPNAg/Btk (98.68% de mortalidade) foi mais eficiente que VPNAg (81.28% de mortalidade) isoladamente, porém o Btk isoladamente causou 100% de mortalidade. Os tratamentos mostraram diferenças significativas (P &lt;0,05) entre AgNPV e Btk,AgNPV e AgNPV/Btk. As análises de patogenicidade do VPNAg e Btk em lagartas de A. gemmatalis sugerem que os produtos Dipel e Baculovirus anticarsia foram mais eficientes, quando utilizados simultaneamente, pois a ação do VPNAg foi intensificada quando utilizada em associação com Btk, provocando alterações no intestino médio das lagartas a partir de 6 horas após os tratamentos. 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J. Microbiol</addtitle><description>The Anticarsia gemmatalis is responsible for the use of chemical insecticides in the soybean culture, causing a significant increase in the costs of farming and a great unbalance in the ecosystem. The use of microbial agents, like Bacillus thuringiensis serovar kurstaki (Btk) and Anticarsia gemmatalis nucleopolyhedrovirus (AgNPV), they are an alternative to chemical control of the pest insects. In the interaction analysis of the entomopathogenic bacteria and virus it is considered important the in vitro action mode of these microbiology control agents. Therefore, the present study aims the histopathological analysis of the A. gemmatalis larvae digestive system after the interaction in vivo of the entomopathogenic Btk and AgNPV, represented the Dipel and Baculovirus anticarsia formulations, respectively. 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When the entomopathogens were used isolating the gut cells alterations were observed only 12 hours after the treatments. A Anticarsia gemmatalis é responsável pelo uso de inseticidas químicos na cultura da soja, ocasionando um significativo aumento nos custos das lavouras e um grande desequilíbrio no ecossistema. O uso de agentes microbianos, como Bacillus thuringiensis sorovar kurstaki (Btk) e Vírus de Poliedrose Nuclear de Anticarsia gemmatalis (VPNAg), é uma alternativa para o controle químico de insetos-praga. Na análise da interação de bactérias e vírus entomopatogênicos, considera-se importante o modo de ação in vitro desses agentes de controle microbiano. Assim, o presente trabalho objetiva a análise histopatológica do sistema digestivo das lagartas de A. gemmatalis, após a interação dos entomopatógenos Btk e VPNAg, representados nas formulações Dipel e Baculovirus anticarsia, respectivamente. 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Therefore, the present study aims the histopathological analysis of the A. gemmatalis larvae digestive system after the interaction in vivo of the entomopathogenic Btk and AgNPV, represented the Dipel and Baculovirus anticarsia formulations, respectively. The evaluations were realized in larvae of 2nd instar, in which the mortality was evaluated daily, and a histopathology was done with collected larvae in time of 1, 3, 6, 12 and 24 hours after the treatments application. The results of the in vivo assays reveal that the treatment using the association of AgNPV-Btk (98.68% of mortality) was more efficient than using AgNPV isolatedly (81.28% of mortality), but the Btk when used isolatedly had a mortality of 100%. The treatments showed significant (P&lt;0.05) differences between AgNPV and Btk,AgNPV and AgNPV/Btk. The histopathological analysis of the AgNPV and Btk in A. gemmatalis larvae suggests that the Dipel and Baculovirus anticarsia products were more efficient when they were used simultaneously, because the action of AgNPV was intensified when used in association with Btk, causing changes in the larvae midgut after 6 hours of treatments. When the entomopathogens were used isolating the gut cells alterations were observed only 12 hours after the treatments. A Anticarsia gemmatalis é responsável pelo uso de inseticidas químicos na cultura da soja, ocasionando um significativo aumento nos custos das lavouras e um grande desequilíbrio no ecossistema. O uso de agentes microbianos, como Bacillus thuringiensis sorovar kurstaki (Btk) e Vírus de Poliedrose Nuclear de Anticarsia gemmatalis (VPNAg), é uma alternativa para o controle químico de insetos-praga. Na análise da interação de bactérias e vírus entomopatogênicos, considera-se importante o modo de ação in vitro desses agentes de controle microbiano. Assim, o presente trabalho objetiva a análise histopatológica do sistema digestivo das lagartas de A. gemmatalis, após a interação dos entomopatógenos Btk e VPNAg, representados nas formulações Dipel e Baculovirus anticarsia, respectivamente. As avaliações foram realizadas com lagartas de 2º ínstar, onde a mortalidade foi avaliada diariamente, e a histopatologia foi realizada com lagartas coletadas nos períodos de 1, 3, 6, 12 e 24 horas após a aplicação dos tratamentos. Os resultados dos ensaios in vivo, revelam que o tratamento referente à associação VPNAg/Btk (98.68% de mortalidade) foi mais eficiente que VPNAg (81.28% de mortalidade) isoladamente, porém o Btk isoladamente causou 100% de mortalidade. Os tratamentos mostraram diferenças significativas (P &lt;0,05) entre AgNPV e Btk,AgNPV e AgNPV/Btk. As análises de patogenicidade do VPNAg e Btk em lagartas de A. gemmatalis sugerem que os produtos Dipel e Baculovirus anticarsia foram mais eficientes, quando utilizados simultaneamente, pois a ação do VPNAg foi intensificada quando utilizada em associação com Btk, provocando alterações no intestino médio das lagartas a partir de 6 horas após os tratamentos. Quando os entomopatógenos foram utilizados isoladamente, as alterações das células intestinais foram observadas apenas 12 horas após a aplicação dos tratamentos.</abstract><cop>São Paulo</cop><pub>Sociedade Brasileira de Microbiologia</pub><doi>10.1590/S1517-83822005000200017</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
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subjects bacteria
bactéria
biological control
controle biológico
Lepidoptera
MICROBIOLOGY
virus
vírus
title Histopathology of Anticarsia gemmatalis Hübner (Lepidoptera; Noctuidae) treated with Nucleopolyhedrovirus and Bacillus thuringiensis serovar kurstaki
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