Ocurrence of Staphylococcus aureus and multiplex pcr detection of classic enterotoxin genes in cheese and meat products

Multiplex PCR was used to investigate the presence of enterotoxins genes (sea, seb, sec, sed and see) and femA gene (specific for Staphylococcus aureus) in coagulase-positive staphylococci (CPS) isolated from cheese and meat products. From 102 CPS isolates, 91 were positive for femA, 10 for sea, 12...

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Published in:Brazilian journal of microbiology 2009-01, Vol.40 (1), p.145-148
Main Authors: Pelisser, Marcia Regina(Universidade Federal de Santa Catarina Centro de Ciências Agrárias Departamento de Ciência e Tecnologia de Alimentos,Universidade do Contestado), Klein, Cátia Silene(EMBRAPA Suínos e Aves), Ascoli, Kelen Regina(EMBRAPA Suínos e Aves), Zotti, Thaís Regina(EMBRAPA Suínos e Aves), Arisi, Ana Carolina Maisonnave(Universidade Federal de Santa Catarina Centro de Ciências Agrárias Departamento de Ciência e Tecnologia de Alimentos)
Format: Article
Language:English
Subjects:
Bacteria
Bacteriology
Cheese
enterotoxinas
enterotoxins
Meat
MICROBIOLOGY
multiplex PCR
PCR multiplex
Polymerase chain reaction
Staphylococcus aureus
Toxins
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Summary:Multiplex PCR was used to investigate the presence of enterotoxins genes (sea, seb, sec, sed and see) and femA gene (specific for Staphylococcus aureus) in coagulase-positive staphylococci (CPS) isolated from cheese and meat products. From 102 CPS isolates, 91 were positive for femA, 10 for sea, 12 for sed and four for see. PCR multiplex foi empregado para investigar a presença de genes de enterotoxinas estafilocócicas (sea, seb, sec, sed e see) e do gene femA, específico para S.aureus, em cepas de estafilococos coagulase positiva (ECP) isoladas de queijos e derivados cárneos. De 102 cepas, 91 foram positivas para femA, 10 para sea, 12 para sed e 4 para see.
ISSN:1517-8382
1678-4405
1678-4405
DOI:10.1590/S1517-83822009000100025