Construction of killer industrial yeast Saccharomyces cerevisiae HAU-1 and its fermentation performance

Saccharomyces cerevisiae HAU-1, a time tested industrial yeast possesses most of the desirable fermentation characteristics like fast growth and fermentation rate, osmotolerance, high ethanol tolerance, ability to ferment molasses, and to ferment at elevated temperatures etc. However, this yeast was...

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Bibliographic Details
Published in:Brazilian journal of microbiology 2010-04, Vol.41 (2), p.477-485
Main Authors: Bajaj, Bijender K.(University of Jammu Department of Biotechnology), Sharma, S.(CCS Haryana Agric. University Department of Microbiology)
Format: Article
Language:English
Subjects:
distillery yeast
Ethanol
ethanol fermentation
Fermentation
killer yeast
MICROBIOLOGY
molasses
Saccharomyces cerevisiae
Yeast
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Summary:Saccharomyces cerevisiae HAU-1, a time tested industrial yeast possesses most of the desirable fermentation characteristics like fast growth and fermentation rate, osmotolerance, high ethanol tolerance, ability to ferment molasses, and to ferment at elevated temperatures etc. However, this yeast was found to be sensitive against the killer strains of Saccharomyces cerevisiae. In the present study, killer trait was introduced into Saccharomyces cerevisiae HAU-1 by protoplast fusion with Saccharomyces cerevisiae MTCC 475, a killer strain. The resultant fusants were characterized for desirable fermentation characteristics. All the technologically important characteristics of distillery yeast Saccharomyces cerevisiae HAU-1 were retained in the fusants, and in addition the killer trait was also introduced into them. Further, the killer activity was found to be stably maintained during hostile conditions of ethanol fermentations in dextrose or molasses, and even during biomass recycling.
ISSN:1517-8382
1678-4405
1678-4405
DOI:10.1590/S1517-83822010000200030