Brewer's spent grain and corn steep liquor as alternative culture medium substrates for proteinase production by Streptomyces malaysiensis AMT-3

Brewer's spent grain and corn steep liquor or yeast extract were used as the sole organic forms for proteinase production by Streptomyces malaysiensis in submerged fermentation. The influence of the C and N concentrations, as well as the incubation periods, were assessed. Eight proteolytic band...

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Bibliographic Details
Published in:Brazilian journal of microbiology 2011-10, Vol.42 (4), p.1384-1389
Main Authors: Nascimento, Rodrigo Pires do, Alves Junior, Nelson, Coelho, Rosalie Reed Rodrigues
Format: Article
Language:English
Subjects:
Agricultural biotechnology
Fermentation
Grain
MICROBIOLOGY
Proteomics
Yeast
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Summary:Brewer's spent grain and corn steep liquor or yeast extract were used as the sole organic forms for proteinase production by Streptomyces malaysiensis in submerged fermentation. The influence of the C and N concentrations, as well as the incubation periods, were assessed. Eight proteolytic bands were detected through gelatin-gel-electrophoresis in the various extracts obtained from the different media and after different incubation periods, with apparent molecular masses of 20, 35, 43, 50, 70, 100, 116 and 212 kDa. The results obtained suggest an opportunity for exploring this alternative strategy for proteinases production by actinomycetes, using BSG and CSL as economically feasible substrates. [PUBLICATION ABSTRACT]
ISSN:1517-8382
1678-4405
1678-4405
DOI:10.1590/S1517-83822011000400020