Comparative analysis of agr groups and virulence genes among subclinical and clinical mastitis Staphylococcus aureus isolates from sheep flocks of the Northeast of Brazil

Staphylococcus aureus is one of the most frequent mastitis causative agents in small ruminants. The expression of most virulence genes of S. aureus is controlled by an accessory gene regulator (agr) locus. This study aimed to ascertain the prevalence of the different agr groups and to evaluate the o...

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Bibliographic Details
Published in:Brazilian journal of microbiology 2013-01, Vol.44 (2), p.493-498
Main Authors: de Almeida, Lara M, de Almeida, Mayra Zilta P R B, de Mendonça, Carla L, Mamizuka, Elsa M
Format: Article
Language:English
Subjects:
Animals
Bacterial Proteins - genetics
Brazil
Carrier State - microbiology
Carrier State - veterinary
Comparative analysis
Genes
Genotype
Mastitis - microbiology
Mastitis - veterinary
MICROBIOLOGY
Milk - microbiology
Research Paper
Sheep
Sheep Diseases - microbiology
Staphylococcal Infections - microbiology
Staphylococcal Infections - veterinary
Staphylococcus aureus - classification
Staphylococcus aureus - genetics
Staphylococcus aureus - isolation & purification
Staphylococcus infections
Trans-Activators - genetics
Virulence Factors - genetics
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Summary:Staphylococcus aureus is one of the most frequent mastitis causative agents in small ruminants. The expression of most virulence genes of S. aureus is controlled by an accessory gene regulator (agr) locus. This study aimed to ascertain the prevalence of the different agr groups and to evaluate the occurrence of encoding genes for cytotoxin, adhesins and toxins with superantigen activity in S. aureus isolates from milk of ewes with clinical and subclinical mastitis in sheep flocks raised for meat production The agr groups I and II were identified in both cases of clinical and subclinical mastitis. Neither the arg groups III and IV nor negative agr were found. The presence of cflA gene was identified in 100% of the isolates. The frequency of hla and lukE-D genes was high - 77.3 and 82.8%, respectively and all isolates from clinical mastitis presented these genes. The sec gene, either associated to tst gene or not, was identified only in isolates from subclinical mastitis. None of the following genes were identified: bbp, ebpS, cna, fnbB, icaA, icaD, bap, hlg, lukM-lukF-PV and se-a-b-d-e.
ISSN:1517-8382
1678-4405
1678-4405
DOI:10.1590/S1517-83822013000200026