Endophytic fungi from Myrcia guianensis at the Brazilian Amazon: distribution and bioactivity

• Published in: Brazilian journal of microbiology 2014-01, Vol.45 (1), p.153-162
• Main Authors: Dos Banhos, Elissandro Fonseca, de Souza, Antonia Queiroz Lima, de Andrade, Juliano Camurça, de Souza, Afonso Duarte Leão, Koolen, Hector Henrique Ferreira, Albuquerque, Patrícia Melchionna
• Format: Article
• Language: English
• Subjects: Anti-Infective Agents - metabolism; Antimicrobial agents; Bacteria - drug effects; Biodiversity; Brazil; Endophytes - classification; Endophytes - isolation & purification; Endophytes - metabolism; Fermentation; Fungi; Fungi - classification; Fungi - drug effects; Fungi - isolation & purification; Metabolites; Microbial Sensitivity Tests; MICROBIOLOGY; Myrtaceae - microbiology; Plant Leaves - microbiology; Plant Roots - microbiology; Plant Stems - microbiology; Research Paper
• ISSN: 1517-8382; 1678-4405; 1678-4405
• DOI: 10.1590/S1517-83822014005000027

Beneficial interactions between plants and microorganisms have been investigated under different ecological, physiological, biochemical, and genetic aspects. However, the systematic exploration of biomolecules with potential for biotechnological products from this interaction still is relatively scarce. Therefore, this study aimed the evaluation of the diversity and antimicrobial activity of the endophytic fungi obtained from roots, stems and leafs of Myrcia guianensis (Myrtaceae) from the Brazilian Amazon. 156 endophytic fungi were isolated and above 80% were identified by morphological examination as belonging to the genera Pestalotiopsis, Phomopsis, Aspergillus, Xylaria, Nectria, Penicillium and Fusarium. Fermented broth of those fungi were assayed for antimicrobial activity and four inhibited the growth of Staphylococcus aureus, Enterococcus faecalis, Candida albicans and Penicillium avellaneum. As the strain named MgRe2.2.3B (Nectria haematococca) had shown the most promising results against those pathogenic strains, its fermented broth was fractioned and only its two low polar fractions demonstrated to be active. Both fractions exhibited a minimum bactericidal concentration of 50 μg.mL(-1) against S. aureus and a minimum fungicidal concentration of 100 μg.mL(-1) against P. avellaneum. These results demonstrate the diversity of fungal genera in M. guianensis and the potential of these endophytic fungi for the production of new antibiotics.