Filamentous fungi and media for cellulase production in solid state cultures

• Published in: Brazilian journal of microbiology 2014-01, Vol.45 (1), p.279-286
• Main Authors: Kilikian, B V, Afonso, L C, Souza, T F C, Ferreira, R G, Pinheiro, I R
• Format: Article
• Language: English
• Subjects: Biosynthesis; Biotechnology - methods; Cellulase; Cellulase - metabolism; Culture Media - chemistry; Dietary Fiber - metabolism; Fungi; Fungi - enzymology; Fungi - growth & development; Glycine max - metabolism; MICROBIOLOGY; Research Paper; Saccharum - metabolism; Sordariales - enzymology; Sordariales - growth & development; Sugarcane; Trichoderma - enzymology; Trichoderma - growth & development
• ISSN: 1517-8382; 1678-4405; 1678-4405
• DOI: 10.1590/S1517-83822014005000028

Cellulase production was evaluated in two reference strains (T. reesei Rut-C30 and T. reesei QM9414), two strains isolated from a sugarcane cultivation area (Trichoderma sp. IPT778 and T. harzianum rifai IPT821) and one strain isolated in a program for biodiversity preservation in São Paulo state (Myceliophthora thermophila M77). Solid state cultures were performed using sugarcane bagasse (C), wheat bran (W) and/or soybean bran (S). The highest FPA was 10.6 U/gdm for M77 in SC (10:90) at 80% moisture, which was 4.4 times higher than production in pure W. C was a strong inducer of cellulase production, given that the production level of 6.1 U/gdm in WC (40:60) was 2.5 times higher than in pure W for strain M77; T. reesei Rut-C30 did not respond as strongly with about 1.6-fold surplus production. S advantageously replaced W, as the surplus production on SC (20:80) was 2.3 times relative to WC (20:80) for M77.