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Filamentous fungi and media for cellulase production in solid state cultures

Cellulase production was evaluated in two reference strains (T. reesei Rut-C30 and T. reesei QM9414), two strains isolated from a sugarcane cultivation area (Trichoderma sp. IPT778 and T. harzianum rifai IPT821) and one strain isolated in a program for biodiversity preservation in São Paulo state (M...

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Published in:Brazilian journal of microbiology 2014-01, Vol.45 (1), p.279-286
Main Authors: Kilikian, B V, Afonso, L C, Souza, T F C, Ferreira, R G, Pinheiro, I R
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description Cellulase production was evaluated in two reference strains (T. reesei Rut-C30 and T. reesei QM9414), two strains isolated from a sugarcane cultivation area (Trichoderma sp. IPT778 and T. harzianum rifai IPT821) and one strain isolated in a program for biodiversity preservation in São Paulo state (Myceliophthora thermophila M77). Solid state cultures were performed using sugarcane bagasse (C), wheat bran (W) and/or soybean bran (S). The highest FPA was 10.6 U/gdm for M77 in SC (10:90) at 80% moisture, which was 4.4 times higher than production in pure W. C was a strong inducer of cellulase production, given that the production level of 6.1 U/gdm in WC (40:60) was 2.5 times higher than in pure W for strain M77; T. reesei Rut-C30 did not respond as strongly with about 1.6-fold surplus production. S advantageously replaced W, as the surplus production on SC (20:80) was 2.3 times relative to WC (20:80) for M77.
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subjects Biosynthesis
Biotechnology - methods
Cellulase
Cellulase - metabolism
Culture Media - chemistry
Dietary Fiber - metabolism
Fungi
Fungi - enzymology
Fungi - growth & development
Glycine max - metabolism
MICROBIOLOGY
Research Paper
Saccharum - metabolism
Sordariales - enzymology
Sordariales - growth & development
Sugarcane
Trichoderma - enzymology
Trichoderma - growth & development
title Filamentous fungi and media for cellulase production in solid state cultures
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