Search for endophytic diazotrophs in barley seeds

Eight endophytic isolates assigned to Pseudomonas, Azospirillum, and Bacillus genera according to pheno-genotypic features were retrieved from barley seeds under selective pressure for nitrogen-fixers. Genetic relationships among related isolates were investigated through RAPD. Six isolates displaye...

Full description

Saved in:
Bibliographic Details
Published in:Brazilian journal of microbiology 2014-04, Vol.45 (2), p.621-625
Main Authors: Zawoznik, Myriam S, Vázquez, Susana C, Díaz Herrera, Silvana M, Groppa, María D
Format: Article
Language:English
Subjects:
Antibiosis
Azospirillum brasilense - classification
Azospirillum brasilense - genetics
Azospirillum brasilense - isolation & purification
Azospirillum brasilense - metabolism
Bacillus - classification
Bacillus - genetics
Bacillus - isolation & purification
Bacillus - metabolism
Barley
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Endophytes - classification
Endophytes - genetics
Endophytes - isolation & purification
Endophytes - metabolism
Environmental Microbiology
Genes
Genotype & phenotype
Hordeum - microbiology
Indoleacetic Acids - metabolism
MICROBIOLOGY
Molecular Typing
Nitrogen
Nitrogen Fixation
Pseudomonas - classification
Pseudomonas - genetics
Pseudomonas - isolation & purification
Pseudomonas - metabolism
Random Amplified Polymorphic DNA Technique
RNA, Ribosomal, 16S - genetics
Seeds
Seeds - microbiology
Sequence Analysis, DNA
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Eight endophytic isolates assigned to Pseudomonas, Azospirillum, and Bacillus genera according to pheno-genotypic features were retrieved from barley seeds under selective pressure for nitrogen-fixers. Genetic relationships among related isolates were investigated through RAPD. Six isolates displayed nitrogen-fixing ability, while all could biosynthesize indolacetic acid in vitro and showed no antibiosis effects against Azospirillum brasilense Az39, a recognized PGPR.
ISSN:1517-8382
1678-4405
1678-4405
DOI:10.1590/S1517-83822014000200033