Isolation and characterization of arsenic resistant bacteria from wastewater

• Published in: Brazilian journal of microbiology 2014-12, Vol.45 (4), p.1309-1315
• Main Authors: Abbas, Syed Zaghum, Riaz, Mehwish, Ramzan, Naseem, Zahid, M Tariq, Shakoori, Farah R, Rafatullah, Mohd
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - metabolism; Arsenic; Arsenic - metabolism; Arsenites - metabolism; Bacteria; Bioremediation; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Drug Resistance, Bacterial; Enterobacter - classification; Enterobacter - drug effects; Enterobacter - growth & development; Enterobacter - isolation & purification; Environmental Microbiology; Hydrogen-Ion Concentration; Klebsiella pneumoniae - classification; Klebsiella pneumoniae - drug effects; Klebsiella pneumoniae - growth & development; Klebsiella pneumoniae - isolation & purification; Microbial Sensitivity Tests; MICROBIOLOGY; Oxidation-Reduction; Proteome - analysis; Ribotyping; RNA, Ribosomal, 16S - genetics; Temperature; Waste Water - microbiology
• ISSN: 1517-8382; 1678-4405; 1678-4405
• DOI: 10.1590/s1517-83822014000400022

The present study proposed the isolation of arsenic resistant bacteria from wastewater. Only three bacterial isolates (MNZ1, MNZ4 and MNZ6) were able to grow in high concentrations of arsenic. The minimum inhibitory concentrations of arsenic against MNZ1, MNZ4 and MNZ6 were 300 mg/L, 300 mg/L and 370 mg/L respectively. The isolated strains showed maximum growth at 37 °C and at 7.0 pH in control but in arsenite stress Luria Bertani broth the bacterial growth is lower than control. All strains were arsenite oxidizing. All strains were biochemically characterized and ribotyping (16S rRNA) was done for the purpose of identification which confirmed that MNZ1 was homologous to Enterobacter sp. while MNZ4 and MNZ6 showed their maximum homology with Klebsiella pneumoniae. The protein profiling of these strains showed in arsenic stressed and non stressed conditions, so no bands of induced proteins appeared in stressed conditions. The bacterial isolates can be exploited for bioremediation of arsenic containing wastes, since they seem to have the potential to oxidize the arsenite (more toxic) into arsenate (less toxic) form.