Assessment of the pathogenicity of cell-culture-adapted Newcastle disease virus strain Komarov

Newcastle disease vaccines hitherto in vogue are produced from embryonated chicken eggs. Egg-adapted mesogenic vaccines possess several drawbacks such as paralysis and mortality in 2-week-old chicks and reduced egg production in the egg-laying flock. Owing to these possible drawbacks, we attempted t...

Full description

Saved in:
Bibliographic Details
Published in:Brazilian journal of microbiology 2015-07, Vol.46 (3), p.861-865
Main Authors: Visnuvinayagam, Sivam, Thangavel, K, Lalitha, N, Malmarugan, S, Sukumar, Kuppannan
Format: Article
Language:English
Subjects:
Animal diseases
Animals
Cell Culture Techniques
Cells, Cultured
Chick Embryo
Chickens - immunology
Chickens - virology
MICROBIOLOGY
Newcastle disease virus - classification
Newcastle disease virus - growth & development
Newcastle disease virus - pathogenicity
Poultry
Poultry Diseases - immunology
Poultry Diseases - prevention & control
Poultry Diseases - virology
Primary Cell Culture
Vaccination
Vaccines
Vaccines, Attenuated - adverse effects
Vaccines, Attenuated - immunology
Vaccines, Attenuated - therapeutic use
Veterinary Microbiology
Viral Vaccines - adverse effects
Viral Vaccines - immunology
Viral Vaccines - therapeutic use
Viruses
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Newcastle disease vaccines hitherto in vogue are produced from embryonated chicken eggs. Egg-adapted mesogenic vaccines possess several drawbacks such as paralysis and mortality in 2-week-old chicks and reduced egg production in the egg-laying flock. Owing to these possible drawbacks, we attempted to reduce the vaccine virulence for safe vaccination by adapting the virus in a chicken embryo fibroblast cell culture (CEFCC) system. Eighteen passages were carried out by CEFCC, and the pathogenicity was assessed on the basis of the mean death time, intracerebral pathogenicity index, and intravenous pathogenicity index, at equal passage intervals. Although the reduction in virulence demonstrated with increasing passage levels in CEFCC was encouraging, 20% of the 2-week-old birds showed paralytic symptoms with the virus vaccine from the 18(th)(final) passage. Thus, a tissue-culture-adapted vaccine would demand a few more passages by CEFCC in order to achieve a complete reduction in virulence for use as a safe and effective vaccine, especially among younger chicks. Moreover, it can be safely administered even to unprimed 8-week-old birds.
ISSN:1678-4405
1517-8382
1678-4405
DOI:10.1590/S1517-838246320140051