Removal and killing of multispecies endodontic biofilms by N-acetylcysteine

Removal of bacterial biofilm from the root canal system is essential for the management of endodontic disease. Here we evaluated the antibacterial effect of N-acetylcysteine (NAC), a potent antioxidant and mucolytic agent, against mature multispecies endodontic biofilms consisting of Actinomyces nae...

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Bibliographic Details
Published in:Brazilian journal of microbiology 2018-01, Vol.49 (1), p.184-188
Main Authors: Choi, Young-Suk, Kim, Cheul, Moon, Ji-Hoi, Lee, Jin-Yong
Format: Article
Language:English
Subjects:
Acetylcysteine
Actinomyces
Antibacterial
Antioxidants
Biofilm removal
Biofilms
Bioluminescence
Calcium
Calcium hydroxide
Chlorhexidine
Dentin
Disease control
Endodontics
MICROBIOLOGY
Multispecies endodontic biofilm
N-acetylcysteine
Root canal medicament
Root canals
Scanning electron microscopy
Short Communication
Slaked lime
Viability
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Summary:Removal of bacterial biofilm from the root canal system is essential for the management of endodontic disease. Here we evaluated the antibacterial effect of N-acetylcysteine (NAC), a potent antioxidant and mucolytic agent, against mature multispecies endodontic biofilms consisting of Actinomyces naeslundii, Lactobacillus salivarius, Streptococcus mutans and Enterococcus faecalis on sterile human dentin blocks. The biofilms were exposed to NAC (25, 50 and 100mg/mL), saturated calcium hydroxide or 2% chlorhexidine solution for 7 days, then examined by scanning electron microscopy. The biofilm viability was measured by viable cell counts and ATP-bioluminescence assay. NAC showed greater efficacy in biofilm cell removal and killing than the other root canal medicaments. Furthermore, 100mg/mL NAC disrupted the mature multispecies endodontic biofilms completely. These results demonstrate the potential use of NAC in root canal treatment.
ISSN:1517-8382
1678-4405
1678-4405
DOI:10.1016/j.bjm.2017.04.003