An investigation of virulence factors of Legionella pneumophila environmental isolates

Nine Legionella pneumophila strains isolated from cooling towers and a standard strain (L. pneumophila serogroup 1, ATCC 33152, Philadelphia 1) were analyzed and compared in terms of motility, flagella structure, ability to form biofilms, enzymatic activities (hemolysin, nucleases, protease, phospho...

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Bibliographic Details
Published in:Brazilian journal of microbiology 2018-01, Vol.49 (1), p.189-199
Main Authors: Arslan-Aydoğdu, Elif Özlem, Kimiran, Ayten
Format: Article
Language:English
Subjects:
Acanthamoeba castellanii
Acid phosphatase
adhesion
Alkaline phosphatase
bacteria
biofilm
Biofilms
carboxylic ester hydrolases
Cooling towers
Enzymatic activity
enzyme activity
Epidemiology
Flagella
flagellum
Hemagglutination
hemolysins
hosts
humans
Legionella pneumophila
Legionnaires' disease bacterium
Lipase
Macrophages
mice
MICROBIOLOGY
Monocytes
Nuclease
nucleases
Pathogenicity
Pathogens
Phosphatase
Phospholipase
Phospholipase A
Phospholipase C
phospholipases
proteinases
reproduction
Research Paper
serotypes
Strains (organisms)
Turkey (country)
Virulence
Virulence factors
Virulence of environmental isolates
virulent strains
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Summary:Nine Legionella pneumophila strains isolated from cooling towers and a standard strain (L. pneumophila serogroup 1, ATCC 33152, Philadelphia 1) were analyzed and compared in terms of motility, flagella structure, ability to form biofilms, enzymatic activities (hemolysin, nucleases, protease, phospholipase A, phospholipase C, acid phosphatase, alkaline phosphatase and lipase), hemagglutination capabilities, and pathogenicity in various host cells (Acanthamoeba castellanii ATCC 30234, mouse peritoneal macrophages and human peripheral monocytes). All the isolates of bacteria appeared to be motile and polar-flagellated and possessed the type-IV fimbria. Upon the evaluation of virulence factors, isolate 4 was found to be the most pathogenic strain, while 6 out of the 9 isolates (the isolates 1, 2, 3, 4, 5, and 7) were more virulent than the ATCC 33152 strain. The different bacterial strains exhibited differences in properties such as adhesion, penetration and reproduction in the hosts, and preferred host type. To our knowledge, this is the first study to compare the virulence of environmental L. pneumophila strains isolated in Turkey, and it provides important information relevant for understanding the epidemiology of L. pneumophila.
ISSN:1517-8382
1678-4405
1678-4405
DOI:10.1016/j.bjm.2017.03.012