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An investigation of virulence factors of Legionella pneumophila environmental isolates
Nine Legionella pneumophila strains isolated from cooling towers and a standard strain (L. pneumophila serogroup 1, ATCC 33152, Philadelphia 1) were analyzed and compared in terms of motility, flagella structure, ability to form biofilms, enzymatic activities (hemolysin, nucleases, protease, phospho...
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Published in: | Brazilian journal of microbiology 2018-01, Vol.49 (1), p.189-199 |
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description | Nine Legionella pneumophila strains isolated from cooling towers and a standard strain (L. pneumophila serogroup 1, ATCC 33152, Philadelphia 1) were analyzed and compared in terms of motility, flagella structure, ability to form biofilms, enzymatic activities (hemolysin, nucleases, protease, phospholipase A, phospholipase C, acid phosphatase, alkaline phosphatase and lipase), hemagglutination capabilities, and pathogenicity in various host cells (Acanthamoeba castellanii ATCC 30234, mouse peritoneal macrophages and human peripheral monocytes). All the isolates of bacteria appeared to be motile and polar-flagellated and possessed the type-IV fimbria. Upon the evaluation of virulence factors, isolate 4 was found to be the most pathogenic strain, while 6 out of the 9 isolates (the isolates 1, 2, 3, 4, 5, and 7) were more virulent than the ATCC 33152 strain. The different bacterial strains exhibited differences in properties such as adhesion, penetration and reproduction in the hosts, and preferred host type. To our knowledge, this is the first study to compare the virulence of environmental L. pneumophila strains isolated in Turkey, and it provides important information relevant for understanding the epidemiology of L. pneumophila. |
doi_str_mv | 10.1016/j.bjm.2017.03.012 |
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All the isolates of bacteria appeared to be motile and polar-flagellated and possessed the type-IV fimbria. Upon the evaluation of virulence factors, isolate 4 was found to be the most pathogenic strain, while 6 out of the 9 isolates (the isolates 1, 2, 3, 4, 5, and 7) were more virulent than the ATCC 33152 strain. The different bacterial strains exhibited differences in properties such as adhesion, penetration and reproduction in the hosts, and preferred host type. To our knowledge, this is the first study to compare the virulence of environmental L. pneumophila strains isolated in Turkey, and it provides important information relevant for understanding the epidemiology of L. pneumophila.</description><identifier>ISSN: 1517-8382</identifier><identifier>ISSN: 1678-4405</identifier><identifier>EISSN: 1678-4405</identifier><identifier>DOI: 10.1016/j.bjm.2017.03.012</identifier><identifier>PMID: 29037504</identifier><language>eng</language><publisher>Brazil: Elsevier Editora Ltda</publisher><subject>Acanthamoeba castellanii ; Acid phosphatase ; adhesion ; Alkaline phosphatase ; bacteria ; biofilm ; Biofilms ; carboxylic ester hydrolases ; Cooling towers ; Enzymatic activity ; enzyme activity ; Epidemiology ; Flagella ; flagellum ; Hemagglutination ; hemolysins ; hosts ; humans ; Legionella pneumophila ; Legionnaires' disease bacterium ; Lipase ; Macrophages ; mice ; MICROBIOLOGY ; Monocytes ; Nuclease ; nucleases ; Pathogenicity ; Pathogens ; Phosphatase ; Phospholipase ; Phospholipase A ; Phospholipase C ; phospholipases ; proteinases ; reproduction ; Research Paper ; serotypes ; Strains (organisms) ; Turkey (country) ; Virulence ; Virulence factors ; Virulence of environmental isolates ; virulent strains</subject><ispartof>Brazilian journal of microbiology, 2018-01, Vol.49 (1), p.189-199</ispartof><rights>2017 Sociedade Brasileira de Microbiologia</rights><rights>Copyright © 2017 Sociedade Brasileira de Microbiologia. 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Published by Elsevier Editora Ltda. 2017 Sociedade Brasileira de Microbiologia</rights><rights>This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c617t-6ea28a0349edc832c4c5d3703d2e70f9e82e4c32cd00e961741f8c8f0a8a4d073</citedby><cites>FETCH-LOGICAL-c617t-6ea28a0349edc832c4c5d3703d2e70f9e82e4c32cd00e961741f8c8f0a8a4d073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790574/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1517838216303033$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,3535,24130,27903,27904,45759,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29037504$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arslan-Aydoğdu, Elif Özlem</creatorcontrib><creatorcontrib>Kimiran, Ayten</creatorcontrib><title>An investigation of virulence factors of Legionella pneumophila environmental isolates</title><title>Brazilian journal of microbiology</title><addtitle>Braz J Microbiol</addtitle><description>Nine Legionella pneumophila strains isolated from cooling towers and a standard strain (L. pneumophila serogroup 1, ATCC 33152, Philadelphia 1) were analyzed and compared in terms of motility, flagella structure, ability to form biofilms, enzymatic activities (hemolysin, nucleases, protease, phospholipase A, phospholipase C, acid phosphatase, alkaline phosphatase and lipase), hemagglutination capabilities, and pathogenicity in various host cells (Acanthamoeba castellanii ATCC 30234, mouse peritoneal macrophages and human peripheral monocytes). All the isolates of bacteria appeared to be motile and polar-flagellated and possessed the type-IV fimbria. Upon the evaluation of virulence factors, isolate 4 was found to be the most pathogenic strain, while 6 out of the 9 isolates (the isolates 1, 2, 3, 4, 5, and 7) were more virulent than the ATCC 33152 strain. The different bacterial strains exhibited differences in properties such as adhesion, penetration and reproduction in the hosts, and preferred host type. To our knowledge, this is the first study to compare the virulence of environmental L. pneumophila strains isolated in Turkey, and it provides important information relevant for understanding the epidemiology of L. pneumophila.</description><subject>Acanthamoeba castellanii</subject><subject>Acid phosphatase</subject><subject>adhesion</subject><subject>Alkaline phosphatase</subject><subject>bacteria</subject><subject>biofilm</subject><subject>Biofilms</subject><subject>carboxylic ester hydrolases</subject><subject>Cooling towers</subject><subject>Enzymatic activity</subject><subject>enzyme activity</subject><subject>Epidemiology</subject><subject>Flagella</subject><subject>flagellum</subject><subject>Hemagglutination</subject><subject>hemolysins</subject><subject>hosts</subject><subject>humans</subject><subject>Legionella pneumophila</subject><subject>Legionnaires' disease 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Özlem</au><au>Kimiran, Ayten</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An investigation of virulence factors of Legionella pneumophila environmental isolates</atitle><jtitle>Brazilian journal of microbiology</jtitle><addtitle>Braz J Microbiol</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>49</volume><issue>1</issue><spage>189</spage><epage>199</epage><pages>189-199</pages><issn>1517-8382</issn><issn>1678-4405</issn><eissn>1678-4405</eissn><abstract>Nine Legionella pneumophila strains isolated from cooling towers and a standard strain (L. pneumophila serogroup 1, ATCC 33152, Philadelphia 1) were analyzed and compared in terms of motility, flagella structure, ability to form biofilms, enzymatic activities (hemolysin, nucleases, protease, phospholipase A, phospholipase C, acid phosphatase, alkaline phosphatase and lipase), hemagglutination capabilities, and pathogenicity in various host cells (Acanthamoeba castellanii ATCC 30234, mouse peritoneal macrophages and human peripheral monocytes). All the isolates of bacteria appeared to be motile and polar-flagellated and possessed the type-IV fimbria. Upon the evaluation of virulence factors, isolate 4 was found to be the most pathogenic strain, while 6 out of the 9 isolates (the isolates 1, 2, 3, 4, 5, and 7) were more virulent than the ATCC 33152 strain. The different bacterial strains exhibited differences in properties such as adhesion, penetration and reproduction in the hosts, and preferred host type. To our knowledge, this is the first study to compare the virulence of environmental L. pneumophila strains isolated in Turkey, and it provides important information relevant for understanding the epidemiology of L. pneumophila.</abstract><cop>Brazil</cop><pub>Elsevier Editora Ltda</pub><pmid>29037504</pmid><doi>10.1016/j.bjm.2017.03.012</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Acanthamoeba castellanii Acid phosphatase adhesion Alkaline phosphatase bacteria biofilm Biofilms carboxylic ester hydrolases Cooling towers Enzymatic activity enzyme activity Epidemiology Flagella flagellum Hemagglutination hemolysins hosts humans Legionella pneumophila Legionnaires' disease bacterium Lipase Macrophages mice MICROBIOLOGY Monocytes Nuclease nucleases Pathogenicity Pathogens Phosphatase Phospholipase Phospholipase A Phospholipase C phospholipases proteinases reproduction Research Paper serotypes Strains (organisms) Turkey (country) Virulence Virulence factors Virulence of environmental isolates virulent strains |
title | An investigation of virulence factors of Legionella pneumophila environmental isolates |
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