Performance of “RESIST-3 O.K.N. K-SeT” immunochromatographic assay for the detection of OXA-48 like, KPC, and NDM carbapenemases in Klebsiella pneumoniae in Turkey

• Published in: Brazilian journal of microbiology 2018-10, Vol.49 (4), p.885-890
• Main Authors: Sağıroğlu, Pınar, Hasdemir, Ufuk, Altınkanat Gelmez, Gülşen, Aksu, Burak, Karatuna, Onur, Söyletir, Güner
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; Antibiotics; Assaying; Bacteria; Bacterial Proteins - analysis; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; beta-Lactamases - analysis; beta-Lactamases - metabolism; Carbapenems - pharmacology; Citrobacter; Cross-reaction; Diagnostic systems; E coli; Enterobacter cloacae; Gram-negative bacteria; Humans; Immunoassay - methods; Immunochromatographic test; Klebsiella; Klebsiella Infections - microbiology; Klebsiella pneumoniae; Klebsiella pneumoniae - chemistry; Klebsiella pneumoniae - drug effects; Klebsiella pneumoniae - enzymology; Klebsiella pneumoniae - isolation & purification; KPC; MICROBIOLOGY; NDM; OXA-48; Polymerase Chain Reaction; Research Paper; Turkey
• ISSN: 1517-8382; 1678-4405; 1678-4405
• DOI: 10.1016/j.bjm.2018.02.002

In this study, the performance of the “RESIST-3 O.K.N. K-SeT” (Coris BioConcept, Gembloux, Belgium) immunochromatographic assay was evaluated in 132 Klebsiella pneumoniae comprising 102 carbapenem resistant and 30 carbapenem susceptible isolates. Genotypically known isolates of Gram negative bacteria (n=22) including various species were also tested by the assay as controls. The isolates tested by the immunochromatographic assay and also were run PCR for blaKPC, blaIMP, blaVIM, blaNDM, and blaOXA-48. The rates of blaNDM, blaOXA-48, and blaKPC in carbapenem resistant isolates were found at 52.9%, 39.2%, and 2.0%, respectively. Both blaNDM and blaOXA-48 were found in six (5.9%) isolates. The results of the assay showed 100% concordance with those obtained by PCR in 132K. pneumoniae. The agreement between the two methods was found to be identical at the isolate level. The assay also correctly detected all genotypically known isolates of Escherichia coli, Serratia marcescens, Citrobacter freundii, Enterobacter cloacae, K. pneumoniae carrying blaKPC, blaNDM, and/or blaOXA-48. On the other hand, the assay did not exhibit any cross-reaction in control isolates harboring blaIMP and blaVIM. We conclude that the RESIST-3 O.K.N. K-SeT is a reliable, rapid, and user friendly test and we recommend it for routine diagnostic laboratories.