Diversity of microcystin-producing genotypes in Brazilian strains of Microcystis (Cyanobacteria)

Microcystis Kützing ex Lemmermann is among the genera of cyanobacteria often associated to toxic blooms with the release of microcystins. A gene cluster codes for microcystin synthetases, which are involved in the biosynthesis of this toxin. The aim of the present study was to investigate the geneti...

Full description

Saved in:
Bibliographic Details
Published in:Brazilian journal of biology 2011-02, Vol.71 (1), p.209-216
Main Authors: Bittencourt-Oliveira, M C, Oliveira, M C, Pinto, E
Format: Article
Language:English
Subjects:
Base Sequence
BIOLOGY
Brazil
Genes, Bacterial - genetics
Genetic Variation - genetics
Genotype
mcyB
microcystin
Microcystins - biosynthesis
Microcystis - classification
Microcystis - genetics
Molecular Sequence Data
Phylogeny
toxin
variants
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Microcystis Kützing ex Lemmermann is among the genera of cyanobacteria often associated to toxic blooms with the release of microcystins. A gene cluster codes for microcystin synthetases, which are involved in the biosynthesis of this toxin. The aim of the present study was to investigate the genetic diversity of the mcyB gene, specifically the B1 module, in Brazilian strains of Microcystis spp. and its microcystin variants. Broad genetic diversity was revealed in this region. From the phylogenetic analysis, three clusters were obtained that were not related to the geographic origin or morphospecies of the strains, nor with the variant of the microcystin produced. A group of strains that did not produce microcystins was found, despite the presence of the mcyB1 fragment. Eight microcystin isoforms were detected: MC-LR, [D-Asp³]-MC-LR, [Asp³]-MC-LR, MC-RR, [Dha7]-MC-LR, MC-LF, MC-LW and [D-Asp³, EtAdda5]-MC-LH, the latter of which is described for the first time in Brazil. Moreover, five other variants were not identified and indicate being new.
ISSN:1519-6984
1678-4375
1678-4375
1519-6984
DOI:10.1590/S1519-69842011000100030