Molecular characterization of Giardia lamblia and risk factors for giardiasis among immunocompromised patients in southern Brazil

• Published in: Brazilian journal of biology 2022, Vol.82, p.e265055-8
• Main Authors: Jeske, S T, Macedo, M R P, Bianchi, T, Leon, Í F, Pinheiro, N B, Borsuk, S, Villela, M
• Format: Article
• Language: English
• Subjects: Amplification; BIOLOGY; Centrifugation; Cultivars; Cysts; Dehydrogenases; Deoxyribonucleic acid; Diarrhea; DNA; Epidemiology; Ethics; Flotation; Gene sequencing; Genes; Genetic testing; Giardia; Giardia lamblia; Giardiasis; Glutamate dehydrogenase; Immunocompromised hosts; Infections; Malabsorption; Malnutrition; Microscopy; Nucleotide sequence; Parasites; Patients; Pets; Questionnaires; Risk analysis; Risk factors; Sewage treatment; Socioeconomic factors; Wastewater treatment; Weight loss
• ISSN: 1519-6984; 1678-4375; 1678-4375
• DOI: 10.1590/1519-6984.265055

Acute Giardia infections often cause diarrhea and stomach upset. Chronic infections can lead to malnutrition, micronutrient deficiencies, malabsorption and weight loss. This study assessed the prevalence of G. lambia infection and assessed associated risk factors among immunocompomised patients undergoing chemotherapeutic treatment in southern Brazil. A total of 110 immunocompromised patients in Pelotas, RS, Brazil, consented to participate in this study and were recruited. Socioeconomic and epidemiological profile of patients was collected by questionnaire. The prevalence for Giardia were determined through microscopy by the centrifugation-flotation technique using stool samples of every patient. In addition, the genetic characterization of the parasite was carried out by amplifying and sequencing the glutamate dehydrogenase (gdh) gene. By microscopy, the prevalence of giardiasis was 17.3% (19/110). Furthermore, the DNA sequences revealed that 7 (36.8%) out of 19 isolates belonged to assemblage B, while 6 of them (31.6%) belonged to assemblage C, 5 (26.3%) to assemblage A and 1 (5.3%) to assemblage D. Risk factors (p ≤ 0.05) for giardiasis were schooling level (OR=8.0 (1.02 - 62.91) sharing a house with more than three people (OR=14.1 (3.77 - 52.51), water sources (OR=38.9 (10.4 - 145.7), sewage treatment (OR=14.2 (3.1 - 65.5), waste destination (OR=7.44 (2.0 - 27.3), owning pets (OR=4.6 (1.0 - 21.2) and cultivating a vegetable garden (OR=4.2 (1.3 - 13.6). The prevalence of G. lamblia in immunocompromised patients was considered elevate with the identification of four assemblage of the parasite (A, B, C and D).