Viability of periodontal ligament cells in selected transport media for avulsed teeth: an in vitro study

To compare the viability of periodontal ligament (PDL) cells stored in Hanks Balanced Salt Solution (HBSS) with those in readily available transport media over a variable period of time. Methods: Periodontal ligament cells harvested from premolars freshly extracted for orthodontic reasons were cultu...

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Bibliographic Details
Published in:Brazilian journal of oral sciences 2023-11, Vol.22, p.e231499
Main Authors: Williams, Adeola T., Denloye, Obafunke O., Popoola, Bamidele O., Adeniji, Johnson A., Adewumi, Olubusuyi M., Akinleye, Toluwanimi E., Akinyamoju, Clara A.
Format: Article
Language:eng ; por
Subjects:
DENTISTRY, ORAL SURGERY & MEDICINE
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Summary:To compare the viability of periodontal ligament (PDL) cells stored in Hanks Balanced Salt Solution (HBSS) with those in readily available transport media over a variable period of time. Methods: Periodontal ligament cells harvested from premolars freshly extracted for orthodontic reasons were cultured for exponential growth. The cells were exposed to egg white, evaporated milk, water and Hanks Balanced Salt Solution (HBSS) at room temperature. Their viability was evaluated after 30 minutes, 1 hour and 3 hours with the tetrazolium salt-based colorimetric assay (MTT assay). Statistical analysis was done using the IBM® SPSS version 23.0 software. Comparison between the Mean Optical Densities (MODs) of the cells stored in HBSS and other media at each time interval was done using the independent t test. Repeated measure ANOVA and Tukey post-hoc test were also carried out to compare the MOD of cells within each medium over time. The level of significance was set at p
ISSN:1677-3225
1677-3225
DOI:10.20396/bjos.v22i00.8671499