Molecular genetic diversity and mating system in sacha inchi progenies1

• Published in: Pesquisa agropecuária tropical 2017-12, Vol.47 (4), p.480-487
• Main Authors: Valente, Mágno Sávio Ferreira, Lopes, Maria Teresa Gomes, Chaves, Francisco Célio Maia, Pantoja, Mozanil Correia, Sousa, Francy Mary Galúcio, Chagas, Edvan Alves
• Format: Article
• Language: English
• Subjects: AGRICULTURE, MULTIDISCIPLINARY; AGRONOMY
• ISSN: 1983-4063; 1983-4063
• DOI: 10.1590/1983-40632017v4749799

ABSTRACT Sacha inchi (Plukenetia volubilis L.) is a species with nutraceutical benefits traditionally consumed by Amazonic indigenous and urban communities. Studies on the genetic diversity and mating system are required to preserve and make the best use of the genetic resources for this species. This study aimed to estimate the genetic diversity and mating system parameters of sacha inchi progenies using Amplified Fragment Length Polymorphism (AFLP) markers. A total of 360 progenies from 30 accessions of sacha inchi were analyzed using three AFLP primers combinations. The percentage variation between and within families and the crossing rate, among other parameters, were estimated. The AFLP markers were efficient in genetically differentiating progenies, revealing 251 (98.82 %) polymorphic fragments. The analysis of molecular variance showed that the strongest variation occurs between progenies (57.16 %). However, the genetic differentiation within progenies was considerable (42.84 %), and could be exploited in breeding programs. The estimated population outcrossing rate was high (0.957), indicating it is a predominantly allogamous species. On the other hand, 33.7 % of the crosses occurred between related individuals. The estimate for biparental crosses revealed that the progenies consisted mainly of half-siblings (66.9 %) and full-siblings (28.8 %). For purposes of breeding and ex situ genetic conservation, sacha inchi seeds should be collected from a large number of parent plants. RESUMO Sacha inchi (Plukenetia volubilis L.) é uma espécie com benefícios nutracêuticos tradicionalmente consumida por populações indígenas e urbanas amazônicas. Estudos de diversidade genética e do sistema reprodutivo são necessários para a conservação e uso dos recursos genéticos da espécie. Objetivou-se estimar parâmetros de diversidade genética e do sistema reprodutivo de sacha inchi, utilizando-se marcadores Amplified Fragment Length Polymorphism (AFLP). Um total de 360 progênies de 30 subamostras da espécie foram analisadas, com três combinações de primers AFL. Foram estimadas, dentre outros parâmetros, a porcentagem de variação entre e dentro de famílias e a taxa de cruzamento. Os marcadores AFLP foram eficientes na diferenciação genética das progênies, revelando 251 (98,82 %) fragmentos polimórficos. A análise de variância molecular mostrou que a maior parte da variação ocorre entre famílias (57,16 %). No entanto, a diferenciação genética dentro destas foi considerável