Chlorogenic acid improves the quality of boar semen processed in Percoll

This study aimed to evaluate if the addition of chlorogenic acid (ChA) to semen extenders improves the quality of cooled boar semen processed in Percoll. The experimental design was randomized blocks (ejaculates) in a 2×3 factorial (with or without Percoll, and three antioxidant systems: a negative...

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Published in:Animal reproduction 2020-01, Vol.17 (1), p.e20190021-e20190021
Main Authors: Rabelo, Stenia Severo, Resende, Carla Oliveira, Pontelo, Thais Preisser, Chaves, Bruna Resende, Pereira, Bárbara Azevedo, da Silva, William Eduardo, Peixoto, Juliano Vogas, Pereira, Luciano José, Zangeronimo, Márcio Gilberto
Format: Article
Language:English
Subjects:
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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Summary:This study aimed to evaluate if the addition of chlorogenic acid (ChA) to semen extenders improves the quality of cooled boar semen processed in Percoll. The experimental design was randomized blocks (ejaculates) in a 2×3 factorial (with or without Percoll, and three antioxidant systems: a negative control, without supplementation, a positive control - vitamin E, and ChA), totaling six treatments and 12 repetitions. ChA and vitamin E (VE) were added at 4.5 mg/ml and 400 μg/ml in extender, respectively. At 0, 48 and 72h of storage at 15ºC, 80 ml insemination doses each containing 2.0 billion sperm cells were submitted to centrifugation in Percoll. The use of Percoll impaired (P0.05) on the cholesterol efflux, but ChA increased (P
ISSN:1984-3143
1806-9614
1984-3143
DOI:10.21451/1984-3143-AR2019-0021