Effect of supplementation of Aloe vera extracts in cold storage media and cryopreservation of domestic cat epididymal spermatozoa

This study evaluated the effect of the extract of at concentrations of 10% and 20% on the cryopreservation of sperm from the epididymis of domestic cats. Epididymal spermatozoa were recovered using the flotation technique and used in the treatments: control (TRIS-egg yolk at 20%), T10% (TRIS plus 10...

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Published in:Animal reproduction 2020-01, Vol.17 (1), p.e20190067-e20190067
Main Authors: Barbosa, Brenna de Sousa, Dos Santos, Fernanda Araujo, do Macêdo, Luãn Barbalho, Izzo, Roberta Gonçalves, Fernandes, Denilsa Pires, Praxedes, Érika Almeida, Silva, Alexandre Rodrigues, Bezerra, Marcelo Barbosa
Format: Article
Language:English
Subjects:
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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Summary:This study evaluated the effect of the extract of at concentrations of 10% and 20% on the cryopreservation of sperm from the epididymis of domestic cats. Epididymal spermatozoa were recovered using the flotation technique and used in the treatments: control (TRIS-egg yolk at 20%), T10% (TRIS plus 10% of extract), and T20% (TRIS plus 20% of extract). The spermatozoa were subjected to 4ºC for 60 minutes, followed by 20 minutes in nitrogen vapors, and stored in a cryogenic cylinder. The samples were thawed at 37°C for 30 seconds. The sperm motility decreased (
ISSN:1984-3143
1806-9614
1984-3143
DOI:10.21451/1984-3143-AR2019-0067