Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China

The lack of genetic diversity is an obstacle for genetic improvement of upland cotton in China; thus, new technologies must be developed to produce more polymorphic molecular markers associated with agricultural traits of the existing resources for breeding. A highly efficient and economical technol...

Full description

Saved in:
Bibliographic Details
Published in:Crop breeding and applied biotechnology 2018-04, Vol.18 (2), p.155-160
Main Authors: Hou, Jinna, An, Sufang, Liu, Yongjuan, Lei, Li, Li, Baoquan
Format: Article
Language:English
Subjects:
AGRONOMY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Breeding
Cotton
Genetic analysis
Genetic diversity
Genetic improvement
Gossypium hirsutum
Markers
New technology
Polymorphism
Primers
Textile industry
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The lack of genetic diversity is an obstacle for genetic improvement of upland cotton in China; thus, new technologies must be developed to produce more polymorphic molecular markers associated with agricultural traits of the existing resources for breeding. A highly efficient and economical technology of sequence-related amplified polymorphism (SRAP) molecular markers with an automated fragment analyzer ABI3500xl was developed, to detect genetic diversity in upland cotton. Using this new strategy, we easily screened for polymorphisms with 7,872 pairs of SRAP primers, and detected 504 polymorphic markers. Of all these, 165 were used for genetic diversity analysis in 128 upland cotton varieties collected nationwide in China. Our method combined a traditional molecular marker development technology with an economical and easy operation strategy for breeders.
ISSN:1518-7853
1984-7033
1984-7033
DOI:10.1590/1984-70332018v18n2a22