Sequencing and gene expression studies for Al tolerance in contrasting genotypes of tropical maize

The aims of this study were to evaluate the homology of the ZmMATE1 and ZmMATE2 gene sequences with those deposited in the international banks of sequences and quantify the differential expression of the genes ZmMATE1, ZmMATE2, and ZmNrat1 in germplasms of landrace and hybrid maize under aluminum (A...

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Bibliographic Details
Published in:Crop breeding and applied biotechnology 2021, Vol.21 (1)
Main Authors: Coelho, Caroline de Jesus, Graczyki, Brenda Luiza, Almeida, Mara Cristina de, Artoni, Roberto Ferreira, Matiello, Rodrigo Rodrigues
Format: Article
Language:English
Subjects:
AGRONOMY
Aluminum
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Corn
Exudation
Gene expression
Gene sequencing
Genes
Genotypes
Homology
Hybrids
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Summary:The aims of this study were to evaluate the homology of the ZmMATE1 and ZmMATE2 gene sequences with those deposited in the international banks of sequences and quantify the differential expression of the genes ZmMATE1, ZmMATE2, and ZmNrat1 in germplasms of landrace and hybrid maize under aluminum (Al) stress in minimal solution. Fifty-two hybrids from different companies and 50 landrace varieties were genotyped for the genes ZmMATE1 and ZmMATE2. For studies of gene expression, the tolerant (H 44 and V 18) and the sensitive (H 22 and V 25) genotypes were exposed to minimal solution containing 4 mg L-1 of Al for different periods (0, 1, 3, 6, 9, 12, 24, and 48 h). The results showed greater differential expression in the tolerant V 18 landrace variety of the gene ZmMATE1, indicating that exudation of citrate may be the main mechanism of Al tolerance in this genotype.
ISSN:1518-7853
1984-7033
1984-7033
DOI:10.1590/1984-70332021v21n1a7