Cytotoxicity and antiviral activity evaluation of Cymbopogon spp hydroethanolic extracts

Cymbopogon citratus and C. nardus are noteworthy among the several existing plant species displaying medicinal properties, due to the potential pharmacological activity of these species, including antiviral, antibacterial, antifungal and anti-trypanosomal activities. The objective of this study was...

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Bibliographic Details
Published in:Brazilian Journal of Pharmaceutical Sciences 2019, Vol.55
Main Authors: Chiamenti, Lisandra, Silva, Francini Pereira da, Schallemberger, Karoline, Demoliner, Meriane, Rigotto, Caroline, Fleck, Juliane Deise
Format: Article
Language:English
Subjects:
Acids
Aluminum
Cellular viability
Cymbopogon citratus/toxicity
Cymbopogon nardus/toxicity
Cytotoxicity
Ethanol
Flavonoids
Hepatitis
Herpes viruses
Human mastadenovirus
Laboratories
Metabolites
Pathogens
PHARMACOLOGY & PHARMACY
Phytochemicals
Plants/extracts
Polyphenols
Reagents
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Summary:Cymbopogon citratus and C. nardus are noteworthy among the several existing plant species displaying medicinal properties, due to the potential pharmacological activity of these species, including antiviral, antibacterial, antifungal and anti-trypanosomal activities. The objective of this study was to carry out in vitro toxicity tests of plant extracts from both species and analyze potential antiviral activity against Human mastadenovirus serotype 5 (HAdV-5). Two cell lines (A549 and VERO) were used and mitochondrial and lysosomal viability were determined by the MTT and neutral red assay, respectively, after two exposure times (24 hours and six days). The aim of these assays was to counteract the behavior of the extracts against the different cell lines and determine their non-toxic concentration range, in order to evaluate possible antiviral activity against HAdV-5. Plaque reduction and inhibition index of viral titer assays were performed using the maximum non-cytotoxic concentrations (MNCC) of each extract. The results indicate MNCC at 625 μg/mL for all extracts, except for Cymbopogon nardus obtained with 80% ethanol (CN80), which showed toxicity at concentrations higher than 312.5 μg/mL. CN80 was the only extract that displayed potential activity against HAdV-5, at a concentration of 75 μg/mL, becoming a candidate for extract fraction purification and/or the isolation of substances related to the observed antiviral activity.
ISSN:2175-9790
1984-8250
2175-9790
DOI:10.1590/s2175-97902019000118063