In vitro kinetics of reticulocyte subtypes: maturation after red blood cell storage in additive solution-1 (AS-1)

ABSTRACT Background: Reticulocytes are immature red blood cells containing RNA remnants. Their population kinetics has been documented under various in vivo and in vitro conditions, including after storage of red blood cells in blood banks. The purpose of this study was to describe the influence of...

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Bibliographic Details
Published in:Hematology, Transfusion and Cell Therapy Transfusion and Cell Therapy, 2018-04, Vol.40 (2), p.143-150
Main Authors: Urbina, Adriana, Palomino, Fernando
Format: Article
Language:English
Subjects:
MEDICINE, GENERAL & INTERNAL
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Summary:ABSTRACT Background: Reticulocytes are immature red blood cells containing RNA remnants. Their population kinetics has been documented under various in vivo and in vitro conditions, including after storage of red blood cells in blood banks. The purpose of this study was to describe the influence of blood bank storage on the kinetics of reticulocyte disappearance by in vitro culturing. Method: Samples of reticulocyte-enriched fractions (Percoll density-gradient) were obtained over different storage times from six red blood cell units stored in additive solution-1 (AS-1). Reticulocyte fractions were then cultured in enriched media at 37 ºC and analyzed by flow cytometry with thiazole orange taking into account hemolysis. Results: Density-gradient enriched reticulocyte fractions were obtain from standard red blood cell units with
ISSN:2531-1379
2531-1387
2531-1387
DOI:10.1016/j.htct.2017.12.002