Phosphorescence quenching and the microcirculation: An automated, multipoint oxygen tension measuring instrument

A new, phosphorescence‐based optical instrument for making oxygen tension (PO2) measurements in individual vessels of microvascular preparations is described. Palladium–porphyrin compounds injected into the systemic vasculature are excited by light and the measured phosphorescence emission lifetime...

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Bibliographic Details
Published in:Review of scientific instruments 1995-10, Vol.66 (10), p.5075-5084
Main Authors: Shonat, Ross D., Richmond, Keith N., Johnson, Paul C.
Format: Article
Language:English
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Summary:A new, phosphorescence‐based optical instrument for making oxygen tension (PO2) measurements in individual vessels of microvascular preparations is described. Palladium–porphyrin compounds injected into the systemic vasculature are excited by light and the measured phosphorescence emission lifetime used to calculate intravascular PO2. Because this technique is relatively new, we have undertaken a rigorous examination of its usefulness and inherent limitations for microvascular studies.
ISSN:0034-6748
1089-7623
DOI:10.1063/1.1146133